Protective Effect of Skipjack Tuna Oil against D-Galactose-Induced Aging in Mice

The objective of this research was to evaluate the anti-aging effect of skipjack tuna oil (STO) on a mouse model of D-galactose-induced aging. Healthy ICR mice of reproductive age were randomly divided into 6 groups (n = 8): blank control, low-, middle- and high-dose STO, aging model and positive control groups. Mice in the control group were subjected to subcutaneous injection of 0.9% saline every day, while mice in other experimental groups were subjected to subcutaneous injection of 4%D-galactose (1 000 mg/kg mb) and administrated with different concentrations of STO (20, 50 and 100 mg/mL) at a dose of 10 mL/kg mb by gavage. Mice in the positive control group were administrated with commercial fish oil (100 mg/kg mb)and those in the blank control and aging model groups were by gavage administrated with an equal volume of normal saline for 6 weeks. The activity of glutathione peroxidase (GSH-Px), total superoxide dismutase (T-SOD) and catalase(CAT), and the content of malondialdehyde (MDA) in the liver and serum of mice were determined. The pathological changes of liver tissues were observed. Results indicated that the liver and spleen indexes in the high-dose and blank control groups were significantly lower than in the model group (P © 2019, China Food Publishing Company. All right reserved.