Screening of Trichoderma reesei Mutants and Optimization of Fermentation Conditions for Higher Cellulase Production

Mutants of Trichoderma reesei RUT-C30 induced by atmospheric and room temperature plasma (ARTP) were screened for cellulase production. Whole-genome sequencing was performed to analyze the mutation types of the selected mutant. Also, we investigated the optimization of fermentation medium and conditions for improved production of cellulase. Mutant JNDY-13 was found to produce the highest cellulase activity. Through shake-f l ask fermentation, the filter paper activity(FPA) of JNDY-13 was 2.21 IU/mL, which was 2.21 times higher than that of T. reesei RUT-C30. The highest FPA of 5.40 IU/mL was achieved through fed-batch fermentation in a 5-L fermentor under optimized conditions. A total of 752 mutations were identified in JNDY-13, and the 18-base pair insertion in the galactokinase gene may account for the increased cellulase activity. © 2019, China Food Publishing Company. All right reserved.