Antibacterial Mechanism of ε-Poly-L-lysine with Different Molecular Weight on Staphylococcus aureus

被引：0

作者：

Liao Y. ^{[1
]}

Wang H. ^{[1
]}

Dong T. ^{[1
]}

Yang P. ^{[1
]}

Tang K. ^{[1
]}

Tan Z. ^{[1
]}

Jia S. ^{[1
]}

机构：

[1] State Key Laboratory of Food Nutrition and Safety, College of Bioengineering, Tianjin University of Science and Technology, Tianjin

来源：

Journal of Chinese Institute of Food Science and Technology | 2021年 / 21卷 / 07期

关键词：

Bacteriostatic mechanism; Different molecular weight; Metabolism; Staphylococcus aureus; Ε-poly-L-lysine;

D O I：

10.16429/j.1009-7848.2021.07.004

中图分类号：

学科分类号：

摘要：

Different molecular weights of ε-PL have different antibacterial activities, but the mechanism that causes this difference is not clear. In this study, Gram-positive bacteria Staphylococcus aureus was selected as the research object. From the aspects of antibacterial activity, metabolic level, and cell wall, scanning electron microscope (SEM), microplate reader, gas chromatography-mass spectrometry (GC-MS) and other instruments analyzed the difference in bacteriostatic activity caused by different molecular weight ε-PL, and explored the mechanism of different molecular weight ε-PL on S. aureus. The experiment results showed that compared with the low-molecular-weight(<1 ku) ε-PL treatment group, the high-molecular-weight ε-PL(1-3 ku and >3 ku) makes the surface of the cells sag and shrink to different degrees. The metabolomics method was used to study the differences in the metabolic levels of S. aureus after the action of different molecular weight ε-PL. The results showed that high molecular weight (1-3 ku and > 3 ku) ε-PL and the initial ε-PL inhibited the glycolytic pathway and tricarboxylic acid cycle much more than the low molecular weight (<1 ku) ε-PL. It has been verified that the bacteriostatic effects to S. aureus with different molecular weight ε-PL are different, which provides theoretical support for the use of preservatives in actual food processing and production processes. © 2021, Editorial Office of Journal of CIFST. All right reserved.

引用

页码：28 / 36

页数：8

共 20 条

[11] VEERMAN E C, VALENTIJN-BENZ M, VAN'T HOF W, Et al., Phytosphingosine kills Candida albicans by disrupting its cell membrane, Biological Chemistry, 391, 1, pp. 65-71, (2010)
[12] ROSENBERG M, GUTNICK D, ROSENBERG E., Adherence of bacteria to hydrocarbons: A simple method for measuring cell-surface hydrophobicity, FEMS Microbiology Letters, 9, 1, pp. 29-33, (1980)
[13] SHIMADA S, ANDOU M M, NAITO N, Et al., Effects of hydrostatic pressure on the ultrastructure and leakage of internal substances in the yeast Saccharomyces cerevisiae, Applied Microbiology and Biotechnology, 40, 1, pp. 123-131, (1993)
[14] HANNA M, MANUEL L, MICHAEL L., A protocol for the investigation of the intracellular Staphylococcus aureus metabolome, Analytical Biochemistry, 401, 2, pp. 250-259, (2010)
[15] DING M Z, ZHOU X, YUAN Y J., Metabolome profiling reveals adaptive evolution of Saccharomyces cerevisiae during repeated vacuum fermentations, Metabolomics, 6, 1, pp. 42-55, (2010)
[16] SHIMA S, MATSUOKA H, IWAMOTO T, Et al., Antimicrobial action of ε-poly-L-lysine, Journal of Antibiotics, 37, 11, pp. 1449-1455, (1984)
[17] 11
[18] NAUMANN D, HELM D, LABISCHINSKI H., Microbiological characterizations by FT-IR spectroscopy, Nature, 351, pp. 81-82, (1991)
[19] HYLDGAARD M, MYGIND T, VAD B S, Et al., The antimicrobial mechanism of action of epsilon-poly-L-lysine, Applied and Environmental Microbiology, 80, 24, pp. 7758-7770, (2014)
[20] HAN P, YUAN Y J., Metabolic profiling as a tool for understanding defense response of Taxus cuspidata cells to shear stress, Biotechnology Progress, 25, 5, pp. 1244-1253, (2009)

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