Isolation and Growth Kinetics of Bogoria Virus from Phlebotomine Sandflies Sampled in Baringo, Kenya

被引：0

作者：

Thiiru, Jane Wambui ^{[1
,2
]}

Langat, Solomon ^{[3
]}

Mulwa, Francis ^{[3
]}

Cinkovich, Stephanie ^{[4
]}

Koka, Hellen ^{[3
]}

Yalwala, Santos ^{[1
]}

Khamadi, Samoel ^{[3
]}

Onguso, Justus ^{[2
]}

Odemba, Nicholas ^{[1
]}

Ngere, Francis ^{[1
]}

Johnson, Jaree ^{[5
]}

Egbo, Timothy ^{[1
]}

Garges, Eric ^{[1
]}

Ojwang, Elly ^{[1
]}

Eyase, Fredrick ^{[1
,3
]}

机构：

[1] US Army Med Res Directorate Africa, Dept Emerging Infect Dis, Nairobi, Kenya

[2] Jomo Kenyatta Univ Agr & Technol, Inst Biotechnol Res, Nairobi, Kenya

[3] Kenya Govt Med Res Ctr, Ctr Virus Res, Nairobi, Kenya

[4] US Armed Forces Hlth Surveillance Div, Global Emerging Infect Surveillance Branch, Silver Spring, MD USA

[5] US Armed Forces Pest Management Board, Silver Spring, MD USA

来源：

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE | 2024年 / 111卷 / 02期

关键词：

ALIGNMENT;

D O I：

10.4269/ajtmh.24-0032

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

Phleboviruses are an emerging threat to public health. Recent surveillance efforts in Kenya have unveiled novel phleboviruses. Despite these efforts, there remain knowledge gaps. This study tested female sandflies from diverse ecological settings in Kenya for arboviruses. Sandfly pools were cultured in Vero-CCL cells. Pools showing reproducible cytopathic effects were subjected to next-generation sequencing, followed by phylogenetic analysis. In vitro, cell kinetics analysis was performed using both Vero-E6 cells and C6/36 mosquito cells. One pool from Baringo, Kenya, tested positive for Bogoria virus (BOGV). The BOGV genome clustered in a single clade with previously obtained BOGV genomes. No significant differences were observed between Vero and C6/36 cell growth kinetics. This study has confirmed the presence of BOGV among sandflies in Baringo Kenya and demonstrated growth in mosquito cells.

引用

页码：412 / 415

页数：4

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