An arteriovenous fistula model: Carotid artery-posterior facial vein fistula in rats

Objective: To describe an alternative arteriovenous fistula (AVF) model involving anastomosis of the common carotid artery (CCA) with the posterior facial vein (PFV).Methods: Twenty-two male Sprague-Dawley rats (age 6-8 weeks) were used to establish the AVF model involving end-to-side anastomosis of PFV and CCA. The peak velocity of the CCA and the diameter of the outflow vein were recorded at 7, 14, and 42 days after the operation using Doppler ultrasound. Pathological examination of the intimal lesions was performed at 14 and 42 days after operation.Results: One rat died within 24 h after surgery related to anesthesia. The patency rates at days 7, 14, and 42 were 85.7%, 81%, and 81%, respectively. The diameter of the carotid artery in rats is approximately 0.8 mm. The diameter of the outflow vein was increased by 1.7-fold and 2.2-fold at 7 days (1.1 +/- 0.118 mm) and 14 days (1.4 +/- 0.073 mm). At 42 days (1.96 +/- 0.101 mm) after operation, the diameter was 3-fold greater compared to the unoperated control rat. The peak systolic flow velocity of the carotid artery at 7 days (593 +/- 17.36 mm/s) and 14 days (767 +/- 13.64 mm/s) after surgery was significantly greater compared to the control rat (314 +/- 15.13 mm/s). The rate of increase was fastest at 7 days and leveled off from 14 to 42 days (875 +/- 26 mm/s) after surgery. At 14 days, the intima area showed a nearly 50-fold increase (230 +/- 9.93 mu m2 x 103) compared to control (area 5 +/- 0.37 mu m2 x 103). Comparing 6 weeks with 2 weeks (280 +/- 10.54 mu m2 x 103) after surgery, the intima area increased 1.2 times.Conclusion: The CCA-PFV fistula in rats is a viable alternative AVF model.