Gastroprotective Effect of Isoferulic Acid Derived from Foxtail Millet Bran against Ethanol-Induced Gastric Mucosal Injury by Enhancing GALNT2 Enzyme Activity

被引:1
|
作者
La, Xiaoqin [1 ,2 ]
He, Xiaoting [3 ]
Liang, Jingyi [4 ]
Zhang, Zhaoyan [1 ]
Li, Hanqing [3 ]
Liu, Yizhi [3 ]
Liu, Ting [1 ]
Li, Zhuoyu [4 ,5 ]
Wu, Changxin [1 ,2 ,6 ]
机构
[1] Shanxi Univ, Inst Biomed Sci, Taiyuan 030006, Peoples R China
[2] Shanxi Univ, Shanxi Prov Key Lab Med Mol Cell Biol, Taiyuan 030006, Peoples R China
[3] Shanxi Univ, Sch Life Sci, Taiyuan 030006, Peoples R China
[4] Shanxi Univ, Inst Biotechnol, Taiyuan 030006, Peoples R China
[5] Shanxi Univ, Key Lab Chem Biol & Mol Engn, Natl Minist Educ, Taiyuan 030006, Peoples R China
[6] Shanxi Prov Key Lab Prevent & Treatment Major Infe, Taiyuan 030006, Peoples R China
基金
中国国家自然科学基金;
关键词
foxtail millet; gastric mucosal injury; isoferulic acid; GALNT2; ethanol; O-GLYCOSYLATION; HCL/ETHANOL; PROTECTION; MUCINS;
D O I
10.3390/nu16132148
中图分类号
R15 [营养卫生、食品卫生]; TS201 [基础科学];
学科分类号
100403 ;
摘要
Excessive alcohol consumption has led to the prevalence of gastrointestinal ailments. Alleviating gastric disorders attributed to alcohol-induced thinning of the mucus layer has centered on enhancing mucin secretion as a pivotal approach. In this study, foxtail millet bran polyphenol BPIS was divided into two components with MW < 200 D and MW > 200 D by molecular interception technology. Combined with MTT, cell morphology observation, and trypan blue staining, isoferulic acid (IFA) within the MW < 200 D fraction was determined as the effective constituent to mitigate ethanol-induced damage of gastric epithelial cells. Furthermore, a Wistar rat model with similar clinical features to alcohol-induced gastric mucosal injury was established. Then, gastric morphological observation, H&E staining, and assessments of changes in gastric hexosamine content and gastric wall binding mucus levels were carried out, and the results revealed that IFA (10 mg/Kg) significantly ameliorated alcohol-induced gastric mucosal damage. Finally, we applied techniques including Co-IP, molecular docking, and fluorescence spectroscopy and found that IFA inhibited the alcohol-induced downregulation of N-acetylgalactosamintransferase 2 (GALNT2) activity related to mucus synthesis through direct interaction with GALNT2 in gastric epithelial cells, thus promoting mucin synthesis. Our study lays a foundation for whole grain dietary intervention tailored to individuals suffering from alcoholic gastric mucosal injury.
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页数:18
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