A Triple Signal Amplification Strategy for Accurate and Ultrasensitive miRNA-21 Detection

被引：4
|
作者：
Zhan, Jiajun [1 ]
Wang, Futing [1 ]
Li, Yujin [1 ,2 ]
Li, Jingxian [1 ]
Xiang, Shi [1 ]
Yang, Yan [1 ]
Chen, Kangfu [4 ]
Yang, Hongfen [3 ]
Cai, Ren [1 ]
机构：
[1] Hunan Univ, Coll Mat Sci & Engn, Coll Chem & Chem Engn, Mol Sci & Biomed Lab,State Key Lab Chemo Biosensin, Changsha 410082, Hunan, Peoples R China
[2] Xinyang Normal Univ, Coll Chem & Chem Engn, Xinyang 464000, Peoples R China
[3] Univ South China, Sch Publ Hlth, Hengyang Med Sch, Hunan Key Lab Typ Environm Pollut & Hlth Hazards, Hengyang 421001, Peoples R China
[4] Northwestern Univ, Dept Biomed Engn, Evanston, IL 60201 USA
来源：
ANALYTICAL CHEMISTRY | 2024年 / 96卷 / 36期
基金：
中国国家自然科学基金;
关键词：
COLORIMETRIC DETECTION; MICRORNA; CANCER; BIOSENSOR; DRIVEN;
D O I：
10.1021/acs.analchem.4c02355
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A triple signal amplification strategy was integrated with a built-in double electrode and external energy storage device to fabricate a novel self-powered biosensor for ultrasensitive detection of miRNA-21. Specifically, DNA tetrahedra and haripin2-glucose oxidase are modified on the surface of the biocathode and bioanode by catalytic hairpin assembly (CHA) to achieve dual signal amplification. Moreover, triple signal amplification is realized by including an external capacitor. Consequently, the as-constructed self-powered biosensor demonstrates a low detection limit of 0.06 fM toward the miRNA-21 assay within the range of 0.1 fM to 10 pM. This study presents a practical and sensitive approach to timely cancer detection.
引用
收藏
页码：14464 / 14470
页数：7
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