Qualitative and Quantitative Analyses of Sialyl O-Glycans in Milk-Derived Sialylglycopeptide Concentrate

被引：2
|
作者：
Higuchi, Junichi [1 ]
Kurogochi, Masaki [2 ]
Yamaguchi, Toshiyuki [1 ]
Fujio, Noriki [1 ]
Mitsuduka, Sho [1 ]
Ishida, Yuko [1 ]
Fukudome, Hirofumi [1 ]
Nonoyama, Noriko [1 ]
Gota, Masayuki [1 ]
Mizuno, Mamoru [2 ]
Sakai, Fumihiko [1 ]
机构：
[1] Megmilk Snow Brand Co Ltd, Milk Sci Res Inst, 1-1-2 Minamidai, Kawagoe, Saitama 3501165, Japan
[2] Noguchi Inst, Lab Glyco Organ Chem, 1-9-7 Kaga, Tokyo, Tokyo 1730003, Japan
来源：
FOODS | 2024年 / 13卷 / 17期
关键词：
sialylglycopeptide; O-glycan; homogeneous O-glycopeptide; BOVINE; GLYCOMACROPEPTIDE; PEPTIDES;
D O I：
10.3390/foods13172792
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
Sialyl glycans have several biological functions. We have previously reported on the preparation and bifidogenic activity of milk-derived sialylglycopeptide (MSGP) concentrate containing sialyl O-glycans. The current study qualitatively and quantitatively analyzed the sialyl O-glycans present in the MSGP concentrate. Notably, our quantitative analysis indicated that a majority of O-glycopeptides in the MSGP concentrate were derived from glycomacropeptides. The concentrate was found to contain mainly three types of sialyl core 1 O-glycans, with the disialyl core 1 O-glycan being the most abundant. We successfully quantified three types of sialyl core 1 O-glycans using a meticulous method that used homogeneous O-glycopeptides as calibration standards. Our results provide valuable insights into assessment strategies for the quality control of O-glycans in dietary products and underscore the potential applications of MSGP concentrate in the food industry and other industries.
引用
收藏
页数：14
相关论文
共 50 条
  • [11] CORE 3-DERIVED O-GLYCANS ARE ESSENTIAL FOR INTESTINAL MUCUS BARRIER FUNCTION
    Xia, Lijun
    METHODS IN ENZYMOLOGY, VOL 479: FUNCTIONAL GLYCOMICS, 2010, 479 : 123 - 141
  • [12] Pregnancy-associated changes in the glycosylation of Tamm-Horsfall glycoprotein -: Expression of sialyl Lewis sequences on core 2 type O-glycans derived from uromodulin
    Easton, RL
    Patankar, MS
    Clark, GF
    Morris, HR
    Dell, A
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (29) : 21928 - 21938
  • [13] Premature Ovarian Failure in Mice with Oocytes Lacking Core 1-Derived O-Glycans and Complex N-Glycans
    Williams, Suzannah A.
    Stanley, Pamela
    ENDOCRINOLOGY, 2011, 152 (03) : 1057 - 1066
  • [14] Loss of Core 1-derived O-Glycans Decreases Breast Cancer Development in Mice
    Song, Kai
    Herzog, Brett H.
    Fu, Jianxin
    Sheng, Minjia
    Bergstrom, Kirk
    McDaniel, J. Michael
    Kondo, Yuji
    McGee, Samuel
    Cai, Xiaofeng
    Li, Ping
    Chen, Hong
    Xia, Lijun
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2015, 290 (33) : 20159 - 20166
  • [15] Variations in Oligosaccharides and N/O-Glycans in Human Milk through the Eight-Month Lactation Period
    Wang, Haiyan
    Zhang, Ximei
    Kang, Peng
    Cui, Xiuxiu
    Hao, Guo
    Wang, Zhongfu
    Han, Bei
    Lv, Xin
    Zhang, Jing
    Ge, Wupeng
    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2022, 70 (44) : 14272 - 14283
  • [16] Gestational diabetes mellitus affects the fucosylation and sialylation levels of N/O-glycans in human milk glycoproteins
    Wang, Xiaoqin
    Li, Zhenhua
    Li, Wenqing
    Li, Cheng
    Liu, Jing
    Lu, Yu
    Fan, Jiangbo
    Ren, Haiyan
    Huang, Linjuan
    Wang, Zhongfu
    CARBOHYDRATE POLYMERS, 2023, 301
  • [17] Loss of intestinal core 1-derived O-glycans causes spontaneous colitis in mice
    Fu, Jianxin
    Wei, Bo
    Wen, Tao
    Johansson, Malin E. V.
    Liu, Xiaowei
    Bradford, Emily
    Thomsson, Kristina A.
    McGee, Samuel
    Mansour, Lilah
    Tong, Maomeng
    McDaniel, J. Michael
    Sferra, Thomas J.
    Turner, Jerrold R.
    Chen, Hong
    Hansson, Gunnar C.
    Braun, Jonathan
    Xia, Lijun
    JOURNAL OF CLINICAL INVESTIGATION, 2011, 121 (04): : 1657 - 1666
  • [18] Core 1-derived O-glycans are essential E-selectin ligands on neutrophils
    Yago, Tadayuki
    Fu, Jianxin
    McDaniel, J. Michael
    Miner, Jonathan J.
    McEver, Rodger P.
    Xia, Lijun
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2010, 107 (20) : 9204 - 9209
  • [19] Temporal Quantitative Proteomics Analysis of Neuroblastoma Cells Treated with Bovine Milk-Derived Extracellular Vesicles Highlights the Anti-Proliferative Properties of Milk-Derived Extracellular Vesicles
    Fonseka, Pamali
    Kang, Taeyoung
    Chee, Sing
    Chitti, Sai V.
    Sanwlani, Rahul
    Ang, Ching-Seng
    Mathivanan, Suresh
    CELLS, 2021, 10 (04)
  • [20] Mature intestinal NK cells are increased by milk-derived concentrate supplementation in rotavirus infected neonatal rats
    Perez-Cano, F. J.
    Marin, S.
    Castell, M.
    Rodriguez-Palmero, M.
    Rivero, M.
    Castellote, C.
    Franch, A.
    ANNALS OF NUTRITION AND METABOLISM, 2007, 51 : 179 - 180
12345