Data of fixed-target pink-beam serial synchrotron crystallography at the Pohang Light Source II

Pink-beam serial synchrotron crystallography (SSX) is beneficial in terms of X-ray flux and overcoming partial reflection compared with SSX using a monochromatic beam. The fixed-target (FT) scanning method can minimize the physical damage on the crystal sample when delivering the crystals to the X-ray interaction point. Additionally, general researchers can easily access the experiment since no specialized sample transfer technology is needed. The fixed-target pink-beam SSX at the 1C beamline at the Pohang Light Source II (PLSII) was previously demonstrated using a newly developed magnetic-based sample holder. The room-temperature structure of glucose isomerase and lysozyme were determined using FT pink-beam SSX. Meanwhile, the SSX dataset for glucose isomerase and lysozyme images containing the high Xray background and multi-crystal hits. These data can be tentatively used to develop an indexing algorithm and practice processing the SX data. This study used detailed information on the diffraction data of fixed-target pink-beam SSX at PLSII to access the raw data and process the information. (c) 2024 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY-NC license ( http://creativecommons.org/licenses/by-nc/4.0/ )