Genome-Wide Characterization and Expression Profiling of the AP2/ERF Gene Family in Fragaria vesca L.

被引:2
|
作者
Wei, Yangfan [1 ]
Kong, Yihan [1 ]
Li, Huiwen [1 ]
Yao, Anqi [1 ]
Han, Jiaxin [1 ]
Zhang, Wenhao [1 ]
Li, Xingguo [1 ]
Li, Wenhui [1 ]
Han, Deguo [1 ]
机构
[1] Northeast Agr Univ, Coll Hort & Landscape Architecture, Key Lab Biol & Genet Improvement Hort Crops Northe, Minist Agr & Rural Affairs,Natl Local Joint Engn R, Harbin 150030, Peoples R China
基金
中国国家自然科学基金;
关键词
Fragaria vesca L; AP2/ERF; cold; abiotic stresses; transcription factor; FLORAL HOMEOTIC GENES; TRANSCRIPTION FACTOR; STRESS TOLERANCE; ARABIDOPSIS; COLD; DOMAIN; OVEREXPRESSION; REGULATOR; EVOLUTION; PROTEINS;
D O I
10.3390/ijms25147614
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The wild strawberry (Fragaria vesca L.; F. vesca) represents a resilient and extensively studied model organism. While the AP2/ERF gene family plays a pivotal role in plant development, its exploration within F. vesca remains limited. In this study, we characterized the AP2/ERF gene family in wild strawberries using the recently released genomic data (F. vesca V6.0). We conducted an analysis of the gene family expansion pattern, we examined gene expression in stem segments and leaves under cold conditions, and we explored its functional attributes. Our investigation revealed that the FvAP2/ERF family comprises 86 genes distributed among four subfamilies: AP2 (17), RAV (6), ERF (62), and Soloist (1). Tandem and segmental duplications significantly contributed to the growth of this gene family. Furthermore, predictive analysis identified several cis-acting elements in the promoter region associated with meristematic tissue expression, hormone regulation, and resistance modulation. Transcriptomic analysis under cold stress unveiled diverse responses among multiple FvAP2/ERFs in stem segments and leaves. Real-time fluorescence quantitative reverse transcription PCR (RT-qPCR) results confirmed elevated expression levels of select genes following the cold treatment. Additionally, overexpression of FvERF23 in Arabidopsis enhanced cold tolerance, resulting in significantly increased fresh weight and root length compared to the wild-type control. These findings lay the foundation for further exploration into the functional roles of FvAP2/ERF genes.
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页数:19
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