Purification and Characterization of α-Mannosidase from Onion, Allium cepa

被引：0

作者：

Narita, Yui ^{[1
]}

Tatara, Yota ^{[2
]}

Hamada, Shigeki ^{[1
]}

Kojima, Kaoru ^{[1
]}

Li, Shuai ^{[1
]}

Yoshida, Takashi ^{[1
]}

机构：

[1] Hirosaki Univ, Dept Biochem & Mol Biol, Fac Agr & Life Sci, 3 Bunkyo, Hirosaki, Aomori 0368561, Japan

[2] Hirosaki Univ, Dept Stress Response Sci, Ctr Adv Med Res, Grad Sch Med, 5 Zaifu Cho, Hirosaki, Aomori 0368562, Japan

来源：

JOURNAL OF APPLIED GLYCOSCIENCE | 2024年 / 71卷 / 01期

关键词：

alpha-Mannosidase; onion; Allium cepa; PENICILLIUM-CITRINUM; N-GLYCANS; TURNOVER; GENOME;

D O I：

10.5458/jag.jag.JAG-2023_0010

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

alpha-Mannosidase (ALMAN) extracted from onion (Allium cepa) was purified by column chromatography such as hydrophobic and gel filtration. ALMAN is an acidic alpha-mannosidase that exhibits maximum activity against pNP-alpha-Man at pH 4.0-5.0 at 50 degrees C. Amino acid sequence analysis of ALMAN was consistent with alpha-mannosidase deduced from Allium cepa transcriptome analysis. The gene alman was amplified by PCR using mRNA extracted from onions, and a full-length gene of 3,054 bp encoding a protein of 1,018 amino acid residues was revealed. ALMAN is classified as Glycoside Hydrolase Family (GH) 38 and showed homology with other plant-derived alpha-mannosidases such as tomato and hot pepper.

引用

页码：33 / 36

页数：4

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