The influence of benzene on the composition, diversity and performance of the anodic bacterial community in glucose-fed microbial fuel cells

被引:1
|
作者
Tyszkiewicz, Natalia [1 ,2 ]
Truu, Jaak [3 ]
Mlynarz, Piotr [2 ]
Pasternak, Grzegorz [1 ]
机构
[1] Wroclaw Univ Sci & Technol, Dept Proc Engn & Technol Polymer & Carbon Mat, Lab Microbial Electrochem Syst, Fac Chem, Wroclaw, Poland
[2] Wroclaw Univ Sci & Technol, Fac Chem, Dept Biochem Mol Biol & Biotechnol, Wroclaw, Poland
[3] Univ Tartu, Inst Mol & Cell Biol, Tartu, Estonia
关键词
MFC; petroleum; biodegradation; microbial biodiversity; metabolomics; DEGRADATION; BIODEGRADATION; HYDROCARBONS; GENERATION; ELECTRODE; BIOFILM; PHENOL; POWER;
D O I
10.3389/fmicb.2024.1384463
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Bioelectrochemical systems offer unique opportunities to remove recalcitrant environmental pollutants in a net positive energy process, although it remains challenging because of the toxic character of such compounds. In this study, microbial fuel cell (MFC) technology was applied to investigate the benzene degradation process for more than 160 days, where glucose was used as a co-metabolite and a control. We have applied an inoculation strategy that led to the development of 10 individual microbial communities. The electrochemical dynamics of MFC efficiency was observed, along with their 1H NMR metabolic fingerprints and analysis of the microbial community. The highest power density of 120 mW/m2 was recorded in the final period of the experiment when benzene/glucose was used as fuel. This is the highest value reported in a benzene/co-substrate system. Metabolite analysis confirmed the full removal of benzene, while the dominance of fermentation products indicated the strong occurrence of non-electrogenic reactions. Based on 16S rRNA gene amplicon sequencing, bacterial community analysis revealed several petroleum-degrading microorganisms, electroactive species and biosurfactant producers. The dominant species were recognised as Citrobacter freundii and Arcobacter faecis. Strong, positive impact of the presence of benzene on the alpha diversity was recorded, underlining the high complexity of the bioelectrochemically supported degradation of petroleum compounds. This study reveals the importance of supporting the bioelectrochemical degradation process with auxiliary substrates and inoculation strategies that allow the communities to reach sufficient diversity to improve the power output and degradation efficiency in MFCs beyond the previously known limits. This study, for the first time, provides an outlook on the syntrophic activity of biosurfactant producers and petroleum degraders towards the efficient removal and conversion of recalcitrant hydrophobic compounds into electricity in MFCs.
引用
收藏
页数:12
相关论文
共 50 条
  • [31] Importance of temperature and anodic medium composition on microbial fuel cell (MFC) performance
    Min, Booki
    Roman, Oscar Benito
    Angelidaki, Irini
    BIOTECHNOLOGY LETTERS, 2008, 30 (07) : 1213 - 1218
  • [32] Importance of temperature and anodic medium composition on microbial fuel cell (MFC) performance
    Booki Min
    Óscar Benito Román
    Irini Angelidaki
    Biotechnology Letters, 2008, 30 : 1213 - 1218
  • [33] Electricity generation and microbial community changes in microbial fuel cells packed with different anodic materials
    Sun, Yanmei
    Wei, Jincheng
    Liang, Peng
    Huang, Xia
    BIORESOURCE TECHNOLOGY, 2011, 102 (23) : 10886 - 10891
  • [34] Effects of proton exchange membrane on the performance and microbial community composition of air-cathode microbial fuel cells
    Lee, Yun-Yeong
    Kim, Tae Gwan
    Cho, Kyung-suk
    JOURNAL OF BIOTECHNOLOGY, 2015, 211 : 130 - 137
  • [35] Application of biocathode in microbial fuel cells: cell performance and microbial community
    Chen, Guo-Wei
    Choi, Soo-Jung
    Lee, Tae-Ho
    Lee, Gil-Young
    Cha, Jae-Hwan
    Kim, Chang-Won
    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2008, 79 (03) : 379 - 388
  • [36] Application of biocathode in microbial fuel cells: cell performance and microbial community
    Guo-Wei Chen
    Soo-Jung Choi
    Tae-Ho Lee
    Gil-Young Lee
    Jae-Hwan Cha
    Chang-Won Kim
    Applied Microbiology and Biotechnology, 2008, 79 : 379 - 388
  • [37] The performance of the microbial fuel cell-coupled constructed wetland system and the influence of the anode bacterial community
    Li, Tingting
    Fang, Zhou
    Yu, Ran
    Cao, Xian
    Song, Hailiang
    Li, Xianning
    ENVIRONMENTAL TECHNOLOGY, 2016, 37 (13) : 1683 - 1692
  • [38] The enhancement of microbial fuel cell performance by anodic bacterial community adaptation and cathodic mixed nickel–copper oxides on a graphene electrocatalyst
    Dena Z. Khater
    R. S. Amin
    M. O. Zhran
    Zeinab K. Abd El-Aziz
    Mohamed Mahmoud
    Helmy M. Hassan
    K. M. El-Khatib
    Journal of Genetic Engineering and Biotechnology, 20
  • [39] Influence of wastewater microbial community on the performance of miniaturized microbial fuel cell biosensor
    Xiao, Nan
    Selvaganapathy, P. Ravi
    Wu, Rong
    Huang, Jinhui Jeanne
    BIORESOURCE TECHNOLOGY, 2020, 302
  • [40] Shaping of bacterial community structure in microbial fuel cells by different inocula
    Mei, Xiaoxue
    Guo, Changhong
    Liu, Bingfeng
    Tang, Yu
    Xing, Defeng
    RSC ADVANCES, 2015, 5 (95): : 78136 - 78141