Vapor-liquid-solid grown silica nanowire based electrochemical glucose biosensor

被引：27

作者：

Murphy-Perez, Eduardo ^{[1
]}

Arya, Sunil K. ^{[1
]}

Bhansali, Shekhar ^{[1
]}

机构：

[1] Univ S Florida, Dept Elect Engn, BioMEMS & Microsyst Lab, Tampa, FL 33620 USA

来源：

ANALYST | 2011年 / 136卷 / 08期

基金：

美国国家科学基金会;

关键词：

ELECTROPHORETIC DEPOSITION; CERAMICS; COMPOSITE; SENSORS; ROUTE; FILM;

D O I：

10.1039/c0an00977f

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Vapor-liquid-solid (VLS) grown silica nanowires (SiO(2)NWs) have been deposited electrophoretically on a gold electrode and utilized for covalent immobilization of glucose oxidase (GOx). Covalent binding has been achieved via 3-aminopropyltriethoxysilane (APTES) modification and N-ethyl-N'-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide chemistry. Scanning electron microscopy, transmission electron microscopy and cyclic voltammetry techniques have been used to characterize SiO2NW and GOx/APTES/SiO2NW/Au bioelectrode. Electrochemical studies reveal that SiO2NW increases the effective electro-active surface area thus resulting in higher loading of enzyme. Response characteristics show linearity in the range of interest 25-300 mg dl(-1), with a detection limit of 11 mg dl(-1), sensitivity: 0.463 mu A (mg dl(-1))(-1) and regression coefficient of 0.992.

引用

页码：1686 / 1689

页数：4

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