Spectrophotometry-Based Oxidative Coupling Method for Determining Thymol Utilizing a Coupling Agent

Developing a spectroscopic approach to assess the medicinal substance thymol was one of the research's objectives. Using an oxidative coupling reaction between thymol solution and N,N-dimethyl-p-phenylenediamine dihydrochloride solution (N,NDMPPDADH) in alkaline media with potassium periodate as an oxidizing agent, the current technology forms a blue-colored soluble product. The wavelength at which a colored product exhibits maximum absorption is 600 nm. According to Beer's law, the concentration range covered by the approach under investigation is 1.25-20.00 mu g/mL of thymol. The specific molar absorbance value of 10725.71 L/mol cm indicates the method's sensitivity. The Sandell significance value was 0.014 mu g/cm2, which represents sensitivity per unit length. The precision of the method is demonstrated to be commendable, and the low relative standard deviation value of 0.16% supports this. This method's accuracy in identifying thymol at such low quantities is demonstrated by its confirmed detection limit of 0.0124 mu g/mL. The developed technique has been useful in screening thymol in pharmaceutical products, with mouthwash being the focus of particular attention. Thymol content in real samples was accurately determined using the approach, as evidenced by the reported 101.13% recovery rate of thymol.