Incorporating the diffusivity gradient term in Brownian dynamics simulations of diffusion

In a Comment to the Editor, ( Biophysical Journal, , 2024) Sko<acute accent>ra raises a concern that the modeling framework implemented in Garner and Molines et al. ( Biophysical Journal, , 2023) neglects a potentially important term in the Brownian dynamics simulation of diffusion. Omission of this diffusivity gradient term may lead to an underestimation of the mean and overestimation of the variance of the cytoplasmic viscosity. In this response, we directly address this concern by incorporating this term into our model and showing that for this data set, its effect is negligible and does not alter the conclusions of this work.