Comprehensive profiling of DNA methylation in Korean patients with colorectal cancer

被引：0

作者：

Shim, Hyeran ^{[1
]}

Jang, Kiwon ^{[2
]}

Bang, Yeong Hak ^{[3
,4
]}

Chu, Hoang Bao Khanh ^{[1
]}

Kang, Jisun ^{[1
]}

Lee, Jin-Young ^{[1
]}

Cho, Sheehyun ^{[1
]}

Lee, Hong Seok ^{[1
]}

Jeon, Jongbum ^{[2
]}

Hwang, Taeyeon ^{[2
]}

Joe, Soobok ^{[2
]}

Lim, Jinyeong ^{[5
]}

Choi, Ji-Hye ^{[5
]}

Joo, Eun Hye ^{[5
]}

Park, Kyunghee ^{[6
]}

Moon, Ji Hwan ^{[6
]}

Han, Kyung Yeon ^{[6
]}

Hong, Yourae ^{[7
]}

Lee, Woo Yong ^{[8
]}

Kim, Hee Cheol ^{[8
]}

Yun, Seong Hyeon ^{[8
]}

Cho, Yong Beom ^{[8
]}

Park, Yoon Ah ^{[8
]}

Huh, Jung Wook ^{[8
]}

Shin, Jung Kyong ^{[8
]}

Pyo, Dae Hee ^{[8
]}

Hong, Hyekyung ^{[8
]}

Lee, Hae-Ock ^{[9
,10
]}

Park, Woong-Yang ^{[3
,5
]}

Yang, Jin Ok ^{[2
]}

Kim, Young-Joon ^{[1
,11
]}

机构：

[1] Yonsei Univ, Coll Life Sci & Biotechnol, Dept Biochem, Seoul 03722, South Korea

[2] Korea Res Inst Biosci & Biotechnol, Korea Bioinformat Ctr KOBIC, Daejeon 34141, South Korea

[3] Sungkyunkwan Univ, Samsung Adv Inst Hlth Sci & Technol SAIHST, Dept Digital Hlth, Seoul 06355, South Korea

[4] Sungkyunkwan Univ, Samsung Med Ctr, Sch Med, Dept Med,Div Hematol Oncol, Seoul 06351, South Korea

[5] Sungkyunkwan Univ, Samsung Adv Inst Hlth Sci & Technol, Dept Hlth Sci & Technol, Seoul 06355, South Korea

[6] Sungkyunkwan Univ, Samsung Genome Inst, Samsung Med Ctr, Sch Med, Seoul 06351, South Korea

[7] Katholieke Univ Leuven, Dept Oncol, B-3000 Leuven, Belgium

[8] Sungkyunkwan Univ, Samsung Med Ctr, Sch Med, Dept Surg, Seoul 06351, South Korea

[9] Catholic Univ Korea, Coll Med, Dept Microbiol, Seoul 06591, South Korea

[10] Catholic Univ Korea, Grad Sch, Dept Biomed & Hlth Sci, Seoul 06591, South Korea

[11] LepiDyne Co Ltd, Seoul 04779, South Korea

来源：

BMB REPORTS | 2024年 / 57卷 / 02期

基金：

新加坡国家研究基金会;

关键词：

Colorectal cancer; CpG island methylator phenotype (CIMP); DNA methylation; MutL homolog 1 ( MLH1 ); MICROSATELLITE INSTABILITY; PHENOTYPE; HYPERMETHYLATION; PROMOTER; PACKAGE;

D O I：

10.5483/BMBRep.2023-0093

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Alterations in DNA methylation play an important pathophysiological role in the development and progression of colorectal cancer. We comprehensively profiled DNA methylation alterations in 165 Korean patients with colorectal cancer (CRC), and conducted an in-depth investigation of cancer-specific methylation patterns. Our analysis of the tumor samples revealed a significant presence of hypomethylated probes, primarily within the gene body regions; few hypermethylated sites were observed, which were mostly enriched in promoter-like and CpG island regions. The CpG Island Methylator PhenotypeHigh (CIMP-H) exhibited notable enrichment of microsatellite instability-high (MSI-H). Additionally, our findings indicated a significant correlation between methylation of the MLH1 gene and MSI-H status. Furthermore, we found that the CIMP-H had a higher tendency to affect the right-side of the colon tissues and was slightly more prevalent among older patients. Through our methylome profile analysis, we successfully verified the methylation patterns and clinical characteristics of Korean patients with CRC. This valuable dataset lays a strong foundation for exploring novel molecular insights and potential therapeutic targets for the treatment of CRC. [BMB Reports 2024; 57(2): 110-115]

引用

页码：110 / 115

页数：6

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