Affordable droplet-based flow analyzer with peristaltic micro-pumps for fluorescent ammonium sensing

被引：0

作者：

Sun, Mingtao ^{[1
]}

Jiang, Yiyu ^{[1
]}

Liang, Wenshan ^{[1
]}

Zeng, Hui ^{[1
]}

Chen, Huiwen ^{[1
]}

Zhang, Min ^{[1
]}

机构：

[1] Guilin Univ Elect Technol, Sch Life & Environm Sci, Guangxi Key Lab Automat Detecting Technol & Instru, Guilin 541004, Guangxi, Peoples R China

来源：

TALANTA OPEN | 2024年 / 10卷

基金：

中国国家自然科学基金;

关键词：

3D printing; Flow injection analysis; Droplet-Based analyzer; Fluorescent ammonium detection; Peristaltic pumps; SEQUENTIAL INJECTION; SEAWATER; FIELD;

D O I：

10.1016/j.talo.2024.100356

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An affordable droplet-based flow analyzer incorporating peristaltic micro-pumps has been developed for fluorescent ammonium sensing. The cost-effective peristaltic micro-pumps, modified using 3D-printing techniques, feature a 3D-printed pump base integrated with a Hall sensor to monitor the rotation of the pump motor. This setup generates a pulse flow instead of a continuous flow, delivering specific volumes (typically between 3 to 4 mu L) of solution with each rotation. By using separate pumps to deliver the aqueous and oil phases, these phases merge to form a droplet flowing stream. The relative standard deviation (RSD) values for droplet volumes range from 3.97 % to 5.99 % (n=50) across different pumps. The analyzer utilizes a reaction between ammonium, ortho-phthalaldehyde, and sulfite to produce a fluorescent derivative, allowing for sensitive detection of low ammonium concentrations. A custom light-emitting diode (LED)-based fluorescence detector has been fabricated using 3D printing, ensuring cost-effective production. The analyzer provides a limit of detection of 0.02 mu M (3 sigma) and an RSD of 0.15 % (n=10, 1 mu M ammonium). This analyzer offers several practical advantages, including reduced reagent consumption and the potential for further development in distributed on-site analysis. The use of 3D printing facilitates rapid prototyping and customization, making the system adaptable to various analytical applications.

引用

页数：7