Development of the SupersonicIEF Method for High-Throughput Charge Variant Analysis

被引：1

作者：

McElroy, Will ^{[1
]}

Heger, Christopher D. ^{[1
]}

机构：

[1] ProteinSimple, Biotechne Brand, Applicat Sci, San Jose, CA 95134 USA

来源：

ELECTROPHORESIS | 2024年 / 45卷 / 21-22期

关键词：

charge heterogeneity; fusion proteins; imaged capillary isoelectric focusing; monoclonal antibodies; platform method;

D O I：

10.1002/elps.202400117

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The analysis of biopharmaceuticals for charge variants occurs from early-stage samples through formulation and process-development optimization. Higher throughput methods allow increased analysis of these samples to facilitate greater understanding of the samples and to better optimize their production and formulation. To enable higher throughput charge variant analysis, a new, rapid platform imaged capillary isoelectric focusing (icIEF) method was optimized to be two to three times faster than standard methods.

引用

页码：1968 / 1975

页数：8

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