Metabolomic and Proteomic Analyses to Reveal the Role of Plant-Derived Smoke Solution on Wheat under Salt Stress

被引:2
|
作者
Komatsu, Setsuko [1 ]
Diniyah, Azzahrah [1 ]
Zhu, Wei [2 ]
Nakano, Masataka [3 ]
Rehman, Shafiq Ur [4 ]
Yamaguchi, Hisateru [5 ]
Hitachi, Keisuke [6 ]
Tsuchida, Kunihiro [6 ]
机构
[1] Fukui Univ Technol, Fac Environm & Informat Sci, Fukui 9108505, Japan
[2] Chinese Acad Sci, Hangzhou Inst Med, Hangzhou 310018, Peoples R China
[3] Kanazawa Univ, Res Ctr Expt Modeling Human Dis, Kanazawa 9208640, Japan
[4] Univ Haripur, Dept Biol, Haripur 22620, Pakistan
[5] Yokkaichi Nursing & Med Care Univ, Dept Med Technol, Yokaichi 5128045, Japan
[6] Fujita Hlth Univ, Ctr Med Sci, Toyoake 4701192, Japan
关键词
metabolomics; proteomics; salt stress; wheat; plant-derived smoke solution; COMPREHENSIVE ANALYSIS; GERMINATION; IDENTIFICATION; SALINITY; COMPOUND;
D O I
10.3390/ijms25158216
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Salt stress is a serious problem, because it reduces the plant growth and seed yield of wheat. To investigate the salt-tolerant mechanism of wheat caused by plant-derived smoke (PDS) solution, metabolomic and proteomic techniques were used. PDS solution, which repairs the growth inhibition of wheat under salt stress, contains metabolites related to flavonoid biosynthesis. Wheat was treated with PDS solution under salt stress and proteins were analyzed using a gel-free/label-free proteomic technique. Oppositely changed proteins were associated with protein metabolism and signal transduction in biological processes, as well as mitochondrion, endoplasmic reticulum/Golgi, and plasma membrane in cellular components with PDS solution under salt stress compared to control. Using immuno-blot analysis, proteomic results confirmed that ascorbate peroxidase increased with salt stress and decreased with additional PDS solution; however, H+-ATPase displayed opposite effects. Ubiquitin increased with salt stress and decreased with additional PDS solution; nevertheless, genomic DNA did not change. As part of mitochondrion-related events, the contents of ATP increased with salt stress and recovered with additional PDS solution. These results suggest that PDS solution enhances wheat growth suppressed by salt stress through the regulation of energy metabolism and the ubiquitin-proteasome system related to flavonoid metabolism.
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页数:18
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