A lysosome-located and rhodamine-based fluorescence probe for recognizing hydrogen polysulfide

Hydrogen polysulfide (H2Sn, n >= 2), as a kind of active sulfur species (RSS), has become a hot topic in RSS. It can regulate the biological activity of many proteins through S-sulfhydrylation of cysteine residues (protein CysSSH), and has a protective effect on cells. Although there have been some studies on hydrogen polysulfide, its production, degradation pathway and regulation mechanism still need further be researched. In presented study, an original lysosome-localized fluorescent probe for determining H2Sn was developed utilizing rhodamine as the fluorogen. The probe used morpholine as the locating unit of lysosomes and chose 2-fluoro-5-nitrobenzoate as the recognizing group. Before adding H2Sn, the proposed probe displayed a spironolactone structure and emitted very weak fluorescence. After adding H2Sn, a conjugated xanthene was formed and the probe demonstrated green fluorescence. When the H2Snconcentration was varied from 6.0x10- 7 mol center dot L- 1 to 10.0x10- 5 mol center dot L- 1, the fluorescence intensity of the probe was linearly dependent on the H2Sn concentration. And the detection limit was 1.5x10- 7mol center dot L- 1. The presented probe owned a fast response speed, good selectivity, excellent sensitivity and broad pH work scope. In addition, the probe had been well utilized to sense endogenic and exogenic H2Snin lysosomes.