Optimization of exopolysaccharide production from the novel Enterococcus species, using statistical design of experiment

被引:0
|
作者
Gaur, Shivani Singh [1 ]
Annapure, Uday S. [1 ,2 ]
机构
[1] Inst Chem Technol, Dept Food & Engn Technol, Mumbai, India
[2] Inst Chem Technol, Jalna, India
来源
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY | 2025年 / 55卷 / 03期
关键词
Optimization; exopolysaccharide; microbiology; response surface methodology; bioprocess; fermentation; PROBIOTIC STRAIN; GROWTH; BIOSYNTHESIS;
D O I
10.1080/10826068.2024.2402337
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The Exopolysaccharide (EPS) producing novel strains of Enterococcus previously isolated from the vaginal source of pregnant women were selected based on ropy structure formation. The two selected strains, E.villorum SB-2 and E.rivorum S22-3, were found to be producing 2.87 g/l and 3.14 g/l EPS, respectively, in the minimal media (M17 media) after 24-hour fermentation under anaerobic condition. Both the strains have probiotic properties and have the potential to be used for industrial applications. The production media and fermentation conditions were optimized to enhance the EPS production using the one-factor method, Placket-Burman factorial designing and Central composite design (CCD) of Response surface methodology (RSM). The most relevant factors affecting the EPS yield were sucrose, yeast extract and pH for E.villorum SB2 and sucrose, yeast extract and magnesium sulfate for the E.rivorum S22-3 as determined by Placket-Burman design, whose concentrations were further optimized using CCD. The optimized fermentation conditions gave the total EPS of 9.76 g/l (4 times the initial production) from E.villorum SB-2 and 7.74 g/l (2.5 times the initial production) from E.rivorum S22-3, respectively, after 36-hour incubation at 37 degrees C. These optimization studies might be helpful during scale-up process for the industrial scale production of these exopolysaccharide. [GRAPHICS]
引用
收藏
页码:297 / 308
页数:12
相关论文
共 50 条
  • [21] Statistical Optimization of Lipase Production from Bacillus Species by Submerged Fermentation
    Aachapa, Sharanappa
    Hombalimath, Veeranna S.
    Kamarddi, Jayshree R.
    Desai, Shivalingsarj, V
    Shet, Anil R.
    Patil, Laxmikant R.
    Patil, Jagadish R.
    BIOSCIENCE BIOTECHNOLOGY RESEARCH COMMUNICATIONS, 2021, 14 (01): : 264 - 269
  • [22] Application of statistical design for the optimization of dextranase production by a novel fungus isolated from Red Sea sponge
    Abdelwahed, Nayera A. M.
    Ahmed, Eman Fadl
    El-Gammal, Eman W.
    Hawas, Usama W.
    3 BIOTECH, 2014, 4 (05) : 533 - 544
  • [23] Modeling and optimization of biopolymer (polyhydroxyalkanoates) production from ice cream residue by novel statistical experimental design
    Lee, KM
    Gilmore, DF
    APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2006, 133 (02) : 113 - 148
  • [24] Modeling and optimization of biopolymer (Polyhydroxyalkanoates) production from ice cream residue by novel statistical experimental design
    Kwang-Min Lee
    David F. Gilmore
    Applied Biochemistry and Biotechnology, 2006, 133
  • [25] Application of statistical design for the optimization of dextranase production by a novel fungus isolated from Red Sea sponge
    Nayera A. M. Abdelwahed
    Eman Fadl Ahmed
    Eman W. El-Gammal
    Usama W. Hawas
    3 Biotech, 2014, 4 : 533 - 544
  • [26] Optimization for novel glucanhydrolase production of Lipomyces starkeyi KSM 22 by statistical design
    Park, JS
    Kim, BH
    Lee, JH
    Seo, ES
    Cho, KS
    Park, HJ
    Kang, HK
    Yoo, SK
    Ha, MS
    Chung, HJ
    Cho, DL
    Day, DF
    Kim, DM
    JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 2003, 13 (06) : 993 - 997
  • [27] Optimization of carotenoid production by Rhodotorula glutinis using statistical experimental design
    Park, PK
    Cho, DH
    Kim, EY
    Chu, KH
    WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2005, 21 (04): : 429 - 434
  • [28] Optimization of carotenoid production by Rhodotorula glutinis using statistical experimental design
    P. K. Park
    D. H. Cho
    E. Y. Kim
    K. H. Chu
    World Journal of Microbiology and Biotechnology, 2005, 21 : 429 - 434
  • [29] Production and Characterization of a Novel Exopolysaccharide from Ramlibacter tataouinensis
    Jivkova, Desislava
    Sathiyanarayanan, Ganesan
    Harir, Mourad
    Hertkorn, Norbert
    Schmitt-Kopplin, Philippe
    Sanhaji, Ghislain
    Fochesato, Sylvain
    Berthomieu, Catherine
    Heyraud, Alain
    Achouak, Wafa
    Santaella, Catherine
    Heulin, Thierry
    MOLECULES, 2022, 27 (21):
  • [30] Statistical optimization of molasses based exopolysaccharide and biomass production by Aureobasidium pullulans MTCC 2195
    Srikanth, S.
    Swathi, M.
    Tejaswini, M.
    Sharmila, G.
    Muthukumaran, C.
    Jaganathan, M. K.
    Tamilarasan, K.
    BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY, 2014, 3 (03) : 7 - 12