A microfluidic chip with integrated plasma separation for sample-to-answer detection of multiple chronic disease biomarkers in whole blood

被引：1

作者：

Zhou, Yuan ^{[1
]}

Cui, Aiping ^{[1
]}

Xiang, Dongliu ^{[1
]}

Wang, Qing ^{[1
]}

Huang, Jin ^{[1
]}

Liu, Jianbo ^{[1
]}

Yang, Xiaohai ^{[1
]}

Wang, Kemin ^{[1
]}

机构：

[1] Hunan Univ, Coll Chem & Chem Engn, State Key Lab Chemo Biosensing & Chemometr, Key Lab Bionanotechnol & Mol Engn Hunan Prov, Changsha 410082, Peoples R China

来源：

TALANTA | 2024年 / 280卷

基金：

中国国家自然科学基金;

关键词：

Microfluidic chip; Plasma separation; Chronic disease; Point-of-care testing; Sample-to-answer;

D O I：

10.1016/j.talanta.2024.126701

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Point-of-care testing of multiple chronic disease biomarkers is crucial for timely intervention and management of chronic diseases. Here, a "sample-to-answer" microfluidic chip was developed for simultaneous detection of multiple chronic disease biomarkers in whole blood by integrating a plasma separation module. The whole detection process is very convenient, i.e., just add whole blood and get the results. The chip successfully achieved the simultaneous detection of total cholesterol, triglycerides, uric acid, and glucose in undiluted whole blood within 21 min, including 6 min for plasma separation and 15 min for enzymatic chromogenic reactions. Moreover, the sensitivity levels of on-chip detection of chronic disease biomarkers can also meet clinically relevant thresholds. The chip is easy to use and has significant potential to improve home self-management of chronic diseases and enhance healthcare outcomes.

引用

页数：8

共 38 条

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