Effects of low temperature air exposure and immersion on antioxidant, immune, intestinal flora and metabolome of Chinese mitten crab (Eriocheir sinensis)

被引:1
|
作者
Deng, Xiangyi [1 ,2 ]
Li, Zhiqiang [1 ,2 ,3 ]
Luo, Liang [2 ]
Wang, Shihui [2 ]
Zhang, Rui [2 ]
Guo, Kun [2 ]
Yang, Yuhong [1 ]
Zhao, Zhigang [2 ]
机构
[1] Northeast Agr Univ, Coll Anim Sci & Technol, Harbin 150030, Peoples R China
[2] Chinese Acad Fishery Sci, Heilongjiang River Fisheries Res Inst, Engn Technol Res Ctr Saline alkaline Water Fisheri, Key Open Lab Cold Water Fish Germplasm Resources &, Harbin 150070, Peoples R China
[3] Huludao Ecol Environm protect Serv Ctr, Huludao 125000, Peoples R China
来源
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS | 2024年 / 52卷
关键词
Eriocheir sinensis; Immersion; Air exposure; Physiologic function; Metabolome; OXIDATIVE STRESS; LITOPENAEUS-VANNAMEI; PARALOMIS-GRANULOSA; RESPONSES; DAMAGE; FISH; TAURINE; SYSTEM;
D O I
10.1016/j.cbd.2024.101319
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The aim of this study was to investigate the effects of immersion on immune enzyme activity, haemolymph index, intestinal microbiome and metabolome of E. sinensis after low temperature air exposure. The results showed that low temperature air exposure induced stress response, which led to hepatopancreas injury and increased membrane permeability, but this situation was reversible and alleviated after immersion. In addition, after exposure to low temperature air, haemolymph metabolism-related substances such as glucose and total cholesterol were significantly different from the initial value (P < 0.05), and gradually returned to the initial level after immersion. The changes of intestinal flora and hepatopancreas metabolism caused by low temperature air exposure did not fully recover after immersion, and its negative effects did not completely disappear. The sequencing results showed that the species composition and diversity of intestinal microorganisms of Chinese mitten crabs were changed after low temperature air exposure and immersion treatment. The relative abundance of Bacteroidetes and Proteobacteria were increased, while the relative abundance of Firmicutes was decreased (P < 0.05). Metabolomics analysis showed that lysine levels increased significantly, taurocholic acid levels decreased significantly, and amino acid metabolism and lipid metabolism balance were disturbed in hepatopancreas of E. sinensis after exposure to low temperature air and immersion (P < 0.05). This study will provide new insights into the recovery mechanism of water immersion on Chinese mitten crabs after exposure to air.
引用
收藏
页数:14
相关论文
共 50 条
  • [41] Effects of Dietary L-TRP on Immunity, Antioxidant Capacity and Intestinal Microbiota of the Chinese Mitten Crab (Eriocheir Sinensis) in Pond Culture
    Hou, Mengna
    Pang, Yangyang
    Niu, Chao
    Zhang, Dongxin
    Zhang, Ying
    Liu, Zhiqiang
    Song, Yameng
    Shi, Aoya
    Chen, Qing
    Zhang, Junyan
    Cheng, Yongxu
    Yang, Xiaozhen
    METABOLITES, 2023, 13 (01)
  • [42] A putative insulin receptor involved in immune response of Chinese mitten crab Eriocheir sinensis
    Wang, L.
    Chen, H.
    Qiu, L.
    Wang, L.
    Song, L.
    ISJ-INVERTEBRATE SURVIVAL JOURNAL, 2021, 18 : 1 - 10
  • [43] Iron regulatory protein is involved in the immune defense of the Chinese mitten crab Eriocheir sinensis
    Yang, Lei
    Li, Xuejie
    Wu, Yaomeng
    Zhang, Jiashun
    Li, Weiwei
    Wang, Qun
    FISH & SHELLFISH IMMUNOLOGY, 2019, 89 : 632 - 640
  • [44] High Plasticity of the Gut Microbiome and Muscle Metabolome of Chinese Mitten Crab ( Eriocheir sinensis ) in Diverse Environments
    Chen, Xiaowen
    Chen, Haihong
    Liu, Qinghua
    Ni, Kangda
    Ding, Rui
    Wang, Jun
    Wang, Chenghui
    JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 2021, 31 (02) : 272 - 281
  • [46] Effects of dietary melatonin on hematological immunity, antioxidant defense and antibacterial ability in the Chinese mitten crab, Eriocheir sinensis
    Yang, Xiaozhen
    Song, Xiaozhe
    Zhang, Cong
    Pang, Yangyang
    Song, Yameng
    Cheng, Yongxu
    Nie, Ling
    Zong, Xin
    AQUACULTURE, 2020, 529
  • [47] Common specific indigenous bacteria reside in the intestinal tract of Chinese mitten crab (Eriocheir sinensis)
    Xia, Wenxu
    Chen, Xiaobing
    Yan, Shuling
    Wang, Yongjie
    AQUACULTURE RESEARCH, 2020, 51 (05) : 1780 - 1788
  • [48] The protective effects of melatonin on oxidative damage and the immune system of the Chinese mitten crab (Eriocheir sinensis) exposed to deltamethrin
    Zhang, Cong
    Zhang, Qian
    Pang, Yangyang
    Song, Xiaozhe
    Zhou, Nan
    Wang, Jiang
    He, Long
    Lv, Jiahuan
    Song, Yameng
    Cheng, Yongxu
    Yang, Xiaozhen
    SCIENCE OF THE TOTAL ENVIRONMENT, 2019, 653 : 1426 - 1434
  • [49] Seasonal dynamics of intestinal microbiota in juvenile Chinese mitten crab (Eriocheir sinensis) in the Yangtze Estuary
    Qin, Ze
    Wang, Sikai
    Wu, Yeling
    Sun, Jinhui
    Zhao, Feng
    FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, 2024, 14
  • [50] EFFECTS OF CRYOPROTECTANT TOXICITY ON EMBRYOS OF THE CHINESE MITTEN CRAB, ERIOCHEIR SINENSIS (DECAPODA, BRACHYURA)
    Huang, Xiaorong
    Zhuang, Ping
    Zhang, Longzhen
    Yao, Zhifeng
    Liu, Ting
    Liu, Jianyi
    Feng, Guangpeng
    CRUSTACEANA, 2011, 84 (03) : 281 - 291