Advances of CRISPR/Cas-based Biosensor in Detection of Food-Borne Pathogens

Rapid and accurate detection methods for food -borne pathogens are essential to ensure food safety and human health. One promising innovation in this area is the clustered regularly interspaced short palindromic repeats/CRISPR -associated systems (CRISPR/Cas) biosensor, which utilizes Cas protein and CRISPR RNA (crRNA) ribonucleo protein to specifically recognize target genes, and converts target signals into detectable physical and chemical signals. The CRISPR/Cas biosensor shows many advantages, such as high specificity, programmability, and ease of use, making it promising to pathogen detection. This paper introduced the principles and characteristics of CRISPR/Cas systems, along with the strategies for signal recognition, amplification, and output based on different CRISPR/Cas biosensors, and their respective applications in food -borne pathogen detection. Furthermore, the construction principles and challenges of multiple biosensors based on CRISPR/Cas were explored, as well as their potential for simultaneous detection of multiple pathogens. Finally, the challenges and future development trends of CRISPR/Cas -based biosensors in rapid pathogen detection were discussed, aiming to provide valuable reference and inspiration for biosensor designers and food safety practitioners.