Exploring antihyperglycemic and histopathological analysis of Oxalis corniculata L. hydroethanolic extract in STZ induced diabetic rats with in silico α-amylase inhibitor assessment
Diabetes mellitus is an epidemic and multifaceted metabolic disorder defined by hyperglycemia, that reduces insulin secretion sensitivity, leading to damage to vital organs. This study aimed to investigate the antioxidant and antidiabetic effects of the hydroethanolic extract of Oxalis corniculata L. (OCHEx) in addition to the hypoglycemic effect of bioactive compounds identified from GC-MS profiling using molecular docking studies to explore possible a-amylase inhibitors. The antioxidant capacity of the OCHEx were determined using in vitro DPPH, ABTS , and total antioxidant assays. Antidiabetic, biochemical, and histopathological studies were performed in an in vivo STZ induced rat model. The OCHEx showed antioxidant activity against DPPH, ABTS, and total antioxidant expressed in IC50 value with 124.56 +/- 2.40 mu g/ml, 159.17 +/- 2.98 mu g/ml and 147.83 +/- 1.91 mu g/ml, respectively. Furthermore, the highest inhibition percentages of alpha-amylase (71.0 +/- 2.8), alpha-glucosidase (74.6 +/- 4.2), and acarbose (92.1 +/- 3.7) were obtained at 200 mu g/ml. OCHEx treatment significantly reduced blood glucose levels and concurrently improved blood insulin levels, lipid profiles, and liver marker enzymes significantly ( p <0.05), with improvement in the pancreas and liver revealed through histopathological studies, indicating favour able effects in mitigating diabetic complications. Furthermore, GC-MS screening identified nine compounds, four of which showed potential as a-amylase inhibitors, as investigated through in silico and ADME studies. Our study concludes that OCHEx is a valuable source of antioxidants and ameliorates the risk of diabetes owing to the presence of potential bioactive compounds.
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SRM Univ, SRM Dent Coll, Dept Biochem, Madras 600089, Tamil Nadu, IndiaSRM Univ, SRM Dent Coll, Dept Biochem, Madras 600089, Tamil Nadu, India
Vadivu, R. Shunmuga
Bakthavatchalam, Senthil
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SRM Inst Sci & Technol, Dept Chem, Ramapuram 600089, Tamil Nadu, IndiaSRM Univ, SRM Dent Coll, Dept Biochem, Madras 600089, Tamil Nadu, India
Bakthavatchalam, Senthil
Rani, Vasthi Gnana
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SRM Inst Sci & Technol, Dept Chem, Ramapuram 600089, Tamil Nadu, IndiaSRM Univ, SRM Dent Coll, Dept Biochem, Madras 600089, Tamil Nadu, India
Rani, Vasthi Gnana
Hirad, Abdurahman Hajinur
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King Saud Univ, Coll Sci, Dept Bot & Microbiol, POB 2455, Riyadh 11451, Saudi ArabiaSRM Univ, SRM Dent Coll, Dept Biochem, Madras 600089, Tamil Nadu, India
Hirad, Abdurahman Hajinur
Wen, Zhi-Hong
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Natl Sun Yat Sen Univ, Dept Marine Biotechnol & Resources, Kaohsiung 80424, Taiwan
Natl Sun Yat Sen Univ, Inst Biopharmaceut Sci, Kaohsiung 804201, TaiwanSRM Univ, SRM Dent Coll, Dept Biochem, Madras 600089, Tamil Nadu, India
Wen, Zhi-Hong
Yuan, Chien-Han
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Kaohsiung Armed Forces Gen Hosp, Dept Otolaryngol, Kaohsiung 80284, Taiwan
Natl Sun Yat Sen Univ, Inst Med Sci & Technol, Kaohsiung 80424, Taiwan
Natl Def Med Ctr, Dept Otolaryngol, Taipei 11490, TaiwanSRM Univ, SRM Dent Coll, Dept Biochem, Madras 600089, Tamil Nadu, India
Yuan, Chien-Han
Vinayagam, Ramachandran
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Yeungnam Univ, Coll Life & Appl Sci, Dept Biotechnol, 280 Daehak-Ro, Gyongsan 38541, Gyeongsangbuk D, South KoreaSRM Univ, SRM Dent Coll, Dept Biochem, Madras 600089, Tamil Nadu, India
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Van Yuzuncu Yil Univ, Dept Pharmaceut Biotechnol, Van, TurkiyeVan Yuzuncu Yil Univ, Dept Pharmaceut Biotechnol, Van, Turkiye
Battal, Abdulhamit
Dogan, Abdulahad
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Van Yuzuncu Yil Univ, Dept Biochem, Van, TurkiyeVan Yuzuncu Yil Univ, Dept Pharmaceut Biotechnol, Van, Turkiye
Dogan, Abdulahad
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Uyar, Ahmet
Demir, Abdulbaki
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Van Yuzuncu Yil Univ, Dept Mol Biol & Genet, Van, TurkiyeVan Yuzuncu Yil Univ, Dept Pharmaceut Biotechnol, Van, Turkiye
Demir, Abdulbaki
Keles, oemer Faruk
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Van Yuzuncu Yil Univ, Dept Pathol, Van, TurkiyeVan Yuzuncu Yil Univ, Dept Pharmaceut Biotechnol, Van, Turkiye
Keles, oemer Faruk
Celik, Ismail
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Van Yuzuncu Yil Univ, Dept Mol Biol & Genet, Van, TurkiyeVan Yuzuncu Yil Univ, Dept Pharmaceut Biotechnol, Van, Turkiye
Celik, Ismail
Baloglu, Mehmet Cengiz
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Kastamonu Univ, Dept Genet & Bioengn, Kastamonu, TurkiyeVan Yuzuncu Yil Univ, Dept Pharmaceut Biotechnol, Van, Turkiye
Baloglu, Mehmet Cengiz
Aslan, Ali
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Van Yuzuncu Yil Univ, Dept Pharmacol, Van, Turkiye
Kyrgyz Turkish Manas Univ, Dept Biol, Bishkek, KyrgyzstanVan Yuzuncu Yil Univ, Dept Pharmaceut Biotechnol, Van, Turkiye