Isolation and sequencing of Orthoflavivirus ilheusense from mosquitoes collected in the Brazilian cerrado, West-Central region, Brazil

被引：0

作者：

Reis, Lucia Aline Moura ^{[1
]}

Neto, Joaquim Pinto Nunes ^{[2
]}

do Nascimento, Bruna Lais Sena ^{[2
]}

de Araujo, Alexandre Azevedo ^{[3
]}

Nogueira, Cristiano Gomes ^{[3
]}

Cavalcante, Karina Ribeiro Leite Jardim ^{[4
]}

Ramos, Daniel Garkauskas ^{[5
]}

Durans, Darlene de Brito Simith ^{[2
]}

Wanzeler, Ana Lucia Monteiro ^{[2
]}

Santos, Maissa Maia ^{[2
]}

Rosa Junior, Jose Wilson ^{[2
]}

Goncalves, Ercilia de Jesus ^{[2
]}

da Silva, Sandro Patroca ^{[2
]}

Cruz, Ana Cecilia Ribeiro ^{[2
]}

da Silva, Eliana Vieira Pinto ^{[2
]}

机构：

[1] State Univ Para, Ctr Biol & Hlth Sci, Grad Program Parasitary Biol Amazon Reg, BR-66095663 Belem, Brazil

[2] Evandro Chagas Inst IEC MS SVSA, Dept Arbovirol & Hemorrhag Fevers, BR-67030000 Ananindeua, Brazil

[3] Giovanni Cysneiros Publ Hlth Lab Lacen GO, BR-74853120 Goiania, Brazil

[4] Gen Coordinat Publ Hlth Labs CGLAB GAB SVSA MS, BR-70723040 Brasilia, Brazil

[5] Brazilian Minist Hlth, Secretariat Hlth Surveillance, BR-70709040 Brasilia, Brazil

来源：

ACTA TROPICA | 2024年 / 257卷

关键词：

Orthoflavivirus ilheusense; Arbovirus; Disease vectors; Mosquito vectors; Zoonosis surveillance; Epidemiological monitoring; CAPUCHIN MONKEYS; VIRUS; ARBOVIRUS; CELL; RESIDENTS;

D O I：

10.1016/j.actatropica.2024.107272

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

The Orthoflavivirus ilheusense (ILHV) is an arbovirus that was first isolated in Brazil in 1944 during an epidemiologic investigation of yellow fever. Is a member of the Flaviviridae family and it belongs to the antigenic complex of the Ntaya virus group. Psorophora ferox is the primary vector of ILHV and this study presents the isolation and phylogenetic analysis of ILHV in a pool of Ps. ferox collected in the state of Goi<acute accent>as in 2021. Viral isolation tests were performed on Vero cells and C6/36 clones. The indirect immunofluorescence test (IFI) was used to confirm the positivity of the sample. The positive sample underwent RT-qPCR, sequencing, and phylogenetic analysis. This is the first report of ILHV circulation in this municipality and presented close relationship between this isolate and another ILHV isolate collected in the city of Bele<acute accent>m (PA).

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页数：10

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