LINC-p21 Regulates Pancreatic β-Cell Function in Type 2 Diabetes Mellitus

被引:0
|
作者
Qian, Zengkun [1 ]
Cui, Fan [1 ]
Mao, Zheng [1 ]
Li, Zhen [1 ]
Yi, Xiayu [1 ]
Zhou, Jingjing [1 ]
Cao, Jinjin [1 ]
Li, Xiaoqin [1 ]
机构
[1] Anhui Univ Sci & Technol, Peoples Hosp Wuhu 1, Dept Clin Lab, Wuhu Hosp, Wuhu 241000, Anhui, Peoples R China
关键词
Type 2 diabetes mellitus; LINC-p21; miR-335-3p; IGF-1; Pancreatic beta-cell; DYSFUNCTION;
D O I
10.1007/s10528-024-10850-1
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
This study aimed to investigate the underlying mechanism and assess the biological role of long intergenic non-coding RNA (LINCRNA)-p21 in type 2 diabetes mellitus (T2DM). LINC-p21 and miR-335-3p expression levels were evaluated in blood from T2DM patients, healthy individuals, and mouse islet beta-cell line MIN6 cells grown in a high glucose environment. Apoptosis-related proteins, iNOS, and IGF-1 were detected in vitro and in vivo. Bioinformatics was used to predict that miR-335-3p had complementary binding sites to IGF-1, and a dual-luciferase reporter confirmed the targeting link between LINC-p21 and miR-335-3p. LINC-p21 was highly expressed in the T2DM serum and cells, and LINC-p21 was significantly associated with T2DM prognosis. In vitro and in vivo dysfunction of beta-cells was reduced by LINC-p21 knockdown. MiR-335-3p and IGF-1 may be potential targets of LINC-p21 and miR-335-3p, respectively, after the prediction of the target of LINC-p21 was verified by dual-luciferase assay. Anti-miR-335-3p made LINC-p21 knockdown function again; however, interference of IGF-1 mRNA restored the function of LINC-p21. The miR-335-3p/IGF-1 axis may have a role in the functional protection of pancreatic beta-cells by LINC-p21 silencing, boosting insulin production, and slowing the course of diabetes.
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页数:21
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