X-ray phase-contrast tomography of cells manipulated with an optical stretcher

被引:0
|
作者
Burchert, Jan-Philipp [1 ,2 ]
Frohn, Jasper [1 ]
Roelleke, Ulrike [1 ]
Bruns, Hendrik [1 ]
Yu, Boram [1 ]
Gleber, Sophie-Charlotte [1 ]
Stange, Roland [3 ]
Busse, Madleen [4 ,5 ]
Osterhoff, Markus [1 ]
Salditt, Tim [1 ,2 ]
Koester, Sarah [1 ,2 ]
机构
[1] Univ Gottingen, Inst X ray Phys, Friedrich Hund Pl 1, D-37077 Gottingen, Germany
[2] Univ Gottingen, Cluster Excellence Multiscale Bioimaging Mol Mach, Gottingen, Germany
[3] RS Zelltech, D-94508 Schollnach, Germany
[4] Tech Univ Munich, Sch Sci, Biomed Phys, Boltzmannstr 11, D-85748 Garching, Germany
[5] Tech Univ Munich, Munich Inst Biomed Engn, Boltzmannstr 11, D-85748 Garching, Germany
关键词
optical stretcher; X-ray phase-contrast tomography; high-resolution imaging; biological cells; X-ray stain; NM BEAM CONFINEMENT; FLUORESCENCE MICROSCOPY; DIFFRACTION; HOLOGRAPHY; SCATTERING; CRYSTALS; TOOL;
D O I
10.1107/S1600577524003618
中图分类号
TH7 [仪器、仪表];
学科分类号
0804 ; 080401 ; 081102 ;
摘要
X-rays can penetrate deeply into biological cells and thus allow for examination of their internal structures with high spatial resolution. In this study, X-ray phase-contrast imaging and tomography is combined with an X-ray-compatible optical stretcher and microfluidic sample delivery. Using this setup, individual cells can be kept in suspension while they are examined with the X-ray beam at a synchrotron. From the recorded holograms, 2D phase shift images that are proportional to the projected local electron density of the investigated cell can be calculated. From the tomographic reconstruction of multiple such projections the 3D electron density can be obtained. The cells can thus be studied in a hydrated or even living state, thus avoiding artifacts from freezing, drying or embedding, and can in principle also be subjected to different sample environments or mechanical strains. This combination of techniques is applied to living as well as fixed and stained NIH3T3 mouse fibroblasts and the effect of the beam energy on the phase shifts is investigated. Furthermore, a 3D algebraic reconstruction scheme and a dedicated mathematical description is used to follow the motion of the trapped cells in the optical stretcher for multiple rotations.
引用
收藏
页码:923 / 935
页数:13
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