Machine Learning-Derived Active Sleep as an Early Predictor of White Matter Development in Preterm Infants

White matter dysmaturation is commonly seen in preterm infants admitted to the neonatal intensive care unit (NICU). Animal research has shown that active sleep is essential for early brain plasticity. This study aimed to determine the potential of active sleep as an early predictor for subsequent white matter development in preterm infants. Using heart and respiratory rates routinely monitored in the NICU, we developed a machine learning -based automated sleep stage classi fi er in a cohort of 25 preterm infants (12 females). The automated classi fi er was subsequently applied to a study cohort of 58 preterm infants (31 females) to extract active sleep percentage over 5 - 7 consecutive days during 29 - 32 weeks of postmenstrual age. Each of the 58 infants underwent high -quality T2 -weighted magnetic resonance brain imaging at term -equivalent age, which was used to measure the total white matter volume. The association between active sleep percentage and white matter volume was examined using a multiple linear regression model adjusted for potential confounders. Using the automated classi fi er with a superior sleep classi fi cation performance [mean area under the receiver operating characteristic curve (AUROC) = 0.87, 95% CI 0.83 - 0.92], we found that a higher active sleep percentage during the preterm period was signi fi cantly associated with an increased white matter volume at term -equivalent age [ beta = 0.31, 95% CI 0.09 - 0.53, false discovery rate (FDR)-adjusted p -value = 0.021]. Our results extend the positive association between active sleep and early brain development found in animal research to human preterm infants and emphasize the potential bene fi t of sleep preservation in the NICU setting.