Previous researchers have identified the barriers faced by international teaching assistants (ITAs), yet few studies have specifically explored these barriers within music disciplines. Teaching assistants at American universities often encounter linguistic and sociocultural challenges, while those working in music schools may face additional obstacles due to the unique nature of the subject. The qualitative research method was used to conduct semi-structured interviews with five individuals who currently were or who had been teaching assistants in music departments of colleges and universities. This study explored the challenges faced by international teaching assistants in terms of communication, language and cultural barriers, differences in teaching methods, and difficulties in balancing learning and teaching. Tutor support became an important resource, but the support resources available varied from university to university. The result of this study highlighted the need for professional music teaching training, regular mentor meetings, and support in the daily work and professional development of international teaching assistants.