Chemo-Enzymatic Generation of Highly Fluorescent Nucleoside Analogs Using Purine-Nucleoside Phosphorylase

被引：1

作者：

Stachelska-Wierzchowska, Alicja ^{[1
]}

Wierzchowski, Jacek ^{[1
]}

机构：

[1] Univ Warmia & Mazury, Dept Phys & Biophys, PL-10719 Olsztyn, Poland

来源：

BIOMOLECULES | 2024年 / 14卷 / 06期

关键词：

purine nucleoside phosphorylase; nucleoside analogs; fluorescence; chemo-enzymatic synthesis; NUCLEIC-ACID; ENZYMATIC-SYNTHESIS; RIBOZYME CATALYSIS; 8-AZAPURINES; DNA; NUCLEOTIDES; 2-AMINOPURINE; NUCLEOBASES; 8-AZA-2'-DEOXYISOGUANOSINE; N-6-ETHENOADENINE;

D O I：

10.3390/biom14060701

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Chemo-enzymatic syntheses of strongly fluorescent nucleoside analogs, potentially applicable in analytical biochemistry and cell biology are reviewed. The syntheses and properties of fluorescent ribofuranosides of several purine, 8-azapurine, and etheno-purine derivatives, obtained using various types of purine nucleoside phosphorylase (PNP) as catalysts, as well as alpha-ribose-1-phosphate (r1P) as a second substrate, are described. In several instances, the ribosylation sites are different to the canonical purine N9. Some of the obtained ribosides show fluorescence yields close to 100%. Possible applications of the new analogs include assays of PNP, nucleoside hydrolases, and other enzyme activities both in vitro and within living cells using fluorescence microscopy.

引用

页数：14

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