Generation of a TSC2 knockout embryonic stem cell line by CRISPR/ Cas9 editing

被引：0
|
作者：
Zhang, Siyao [1 ,2 ]
Fan, Jiaqi [1 ,2 ]
Sun, Hairui [1 ,2 ]
Hao, Xiaoyan [1 ,2 ]
He, Yihua [2 ]
机构：
[1] Capital Med Univ, Anzhen Hosp, Beijing 100029, Peoples R China
[2] Capital Med Univ, Beijing Anzhen Hosp, Maternal Fetal Med Ctr Fetal Heart Dis, Beijing 100029, Peoples R China
来源：
STEM CELL RESEARCH | 2024年 / 77卷
基金：
中国国家自然科学基金;
关键词：
D O I：
10.1016/j.scr.2024.103399
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
Tuberous Sclerosis Complex (TSC) is a severe developmental disorder with various clinical effects, primarily caused by TSC2 gene mutations, often involving loss of function(Henske,et al., 2016).To explore role of TSC2 in human heart development, we successfully developed a TSC2 knockout (TSC2-/-) human embryonic stem cells (hESCs) line using CRISPR/Cas9 gene editing. This TSC2-/- hESC line maintained a normal karyotype, expressed pluripotency markers strongly, and could differentiate into all three germ layers in vivo. This cell line will be a valuable tool for future research on the role of TSC2 in heart development.
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页数：5
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