In vivo measurement of the biomechanical properties of human skin with motion-corrected Brillouin microscopy

Biomechanical testing of human skin in vivo is important to study the aging process *maria romodina@mpl mpg de and pathological conditions such as skin cancer. Brillouin microscopy allows the all -optical, Abstract: Biomechancal tesing of human skin in vvo is mportant o tudy th aging non -contact visualization of the mechanical properties of cells and tissues ove r space. Here, process and pathological conditions such as skin cancer Brillouin microscopy allows the all we use the combination of Brillouin microscopy and optical coherence tomography for motioncorrected, depth -resolved biomechanical testing of human skin in vivo. We obtained two peaks ti l, o c t i li ti f th hani l p ti f ll d ti p in the Brillouin spectra for the epidermis, the first at 7 GHz and the second near 9-10 GHz. He , u h co bi i n of Brillo in i py d opic l ohe e g phy for The experimentally measured Brillouin frequency shift of the dermis is lower compared to the motion correced, depth- solved bomech ical testing of human sk epidermis and is 6.8 GHz, indicating the lower stiffness of the dermis.