IS607 TnpB is a hypercompact RNA-guided DNA endonuclease

被引：0
|
作者：
Yang, Hui [1 ]
Patel, Dinshaw J. [2 ]
机构：
[1] Univ Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, Chinese Acad Sci, Ctr Excellence Mol Cell Sci,Key Lab RNA Innovat Sc, Shanghai, Peoples R China
[2] Mem Sloan Kettering Canc Ctr, Struct Biol Program, New York, NY 10021 USA
来源：
CELL RESEARCH | 2024年 / 34卷 / 07期
关键词：
D O I：
10.1038/s41422-024-00973-w
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
The Zhiwei Huang lab report on a hypercompact IS607 TnpB protein that naturally co-occurs with the conserved RAGATH-18 RNA, thereby identifying a new family of compact RNA-guided DNA endonucleases. Such an IS607 TnpB-based approach for programmable genome editing in both Escher-ichia coli and human cells, combined with its structural and target-adjacent motif divergence from earlier reported IS200/IS605 TnpB systems, highlights the diversity of the non-Cas genome editor toolbox.
引用
收藏
页码：473 / 474
页数：2
相关论文
共 50 条
  • [21] Cas9-a programmable RNA-guided DNA endonuclease from the bacterial adaptive immune system
    Gasiunas, G.
    Karvelis, T.
    Barrangou, R.
    Horvath, P.
    Siksnys, V.
    FEBS JOURNAL, 2013, 280 : 80 - 80
  • [22] Creation of targeted inversion mutations in plants using an RNA-guided endonuclease
    Zhang, Congsheng
    Liu, Changlin
    Weng, Jianfeng
    Cheng, Beijiu
    Liu, Fang
    Li, Xinhai
    Xie, Chuanxiao
    CROP JOURNAL, 2017, 5 (01): : 83 - 88
  • [23] Creation of targeted inversion mutations in plants using an RNA-guided endonuclease
    Congsheng Zhang
    Changlin Liu
    Jianfeng Weng
    Beijiu Cheng
    Fang Liu
    Xinhai Li
    Chuanxiao Xie
    TheCropJournal, 2017, 5 (01) : 83 - 88
  • [24] Decrosslinking enables visualization of RNA-guided endonuclease-in situ labeling signals for DNA sequences in plant tissues
    Nagaki, K.
    Yamaji, N.
    JOURNAL OF EXPERIMENTAL BOTANY, 2020, 71 (06) : 1792 - 1800
  • [25] Structure and specificity of the RNA-guided endonuclease Cas9 during DNA interrogation, target binding and cleavage
    Josephs, Eric A.
    Kocak, D. Dewran
    Fitzgibbon, Christopher J.
    McMenemy, Joshua
    Gersbach, Charles A.
    Marszalek, Piotr E.
    NUCLEIC ACIDS RESEARCH, 2015, 43 (18) : 8924 - 8941
  • [26] DNA Targeting by a Minimal CRISPR RNA-Guided Cascade
    Hochstrasser, Megan L.
    Taylor, David W.
    Kornfeld, Jack E.
    Nogales, Eva
    Doudna, Jennifer A.
    MOLECULAR CELL, 2016, 63 (05) : 840 - 851
  • [27] RNA-guided recombination
    Song, Yiyun
    NATURE CHEMICAL BIOLOGY, 2024, 20 (08) : 941 - 941
  • [28] RNA-guided endonuclease provides a therapeutic strategy to cure latent herpesviridae infection
    Wang, Jianbin
    Quake, Stephen R.
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2014, 111 (36) : 13157 - 13162
  • [29] Fanzors, a family of eukaryotic RNA-guided DNA endonucleases
    Jiang, Kaiyi
    Gootenberg, Jonathan S.
    Abudayyeh, Omar O.
    FEBS LETTERS, 2025,
  • [30] Structures guided insight into mechanisms of RNA-guided surveillance of foreign DNA
    Wiedenheft, Blake
    JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS, 2015, 33 : 60 - 61
12345