MOLECULAR-CLONING AND MAPPING OF PHENOL DEGRADATION GENES FROM BACILLUS-STEAROTHERMOPHILUS FDTP-3 AND THEIR EXPRESSION IN ESCHERICHIA-COLI

被引：39
|
作者：
DONG, FM [1 ]
WANG, LL [1 ]
WANG, CM [1 ]
CHENG, JP [1 ]
HE, ZQ [1 ]
SHENG, ZJ [1 ]
SHEN, RQ [1 ]
机构：
[1] FUDAN UNIV,INST GENET,SHANGHAI 200433,PEOPLES R CHINA
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 1992年 / 58卷 / 08期
关键词：
D O I：
10.1128/AEM.58.8.2531-2535.1992
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Two genes of the meta pathway of phenol degradation were cloned from a phenol-utilizing strain of Bacillus stearothermophilus and were mapped by subcloning and by use of a Tn5 insertion mutation. They code for phenol hydroxylase and catechol 2,3-dioxygenase, respectively. The gene encoding catechol 2,3-dioxygenase, which is more thermostable than catechol 2,3-dioxygenase encoded by the other gene, shares rather limited homology with that from Pseudomonas putida.
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页码：2531 / 2535
页数：5
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