NUCLEOSIDE TRANSPORT IN CULTURED LLC-PK1 EPITHELIA

被引:18
|
作者
GRIFFITH, DA [1 ]
DOHERTY, AJ [1 ]
JARVIS, SM [1 ]
机构
[1] UNIV KENT,BIOL LAB,CANTERBURY CT2 7NJ,KENT,ENGLAND
基金
英国医学研究理事会;
关键词
NUCLEOSIDE TRANSPORT; FACILITATED TRANSPORT; ACTIVE TRANSPORT; NITROBENZYLTHIOINOSINE; LLC-PK1; EPITHELIUM; (PIG KIDNEY BRUSH-BORDER VESICLE);
D O I
10.1016/0005-2736(92)90010-J
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The transport of nucleosides by LLC-PK1 cells, a continuous epithelial cell line derived from pig kidney, was characterised. Uridine influx was saturable (apparent K(m) approximately 34-mu-M at 22-degrees-C) and inhibited by > 95% by nitrobenzylthioinosine (NBMPR), dilazep and a variety of purine and pyrimidine nucleosides. In contrast to other cultured animal cells, the NBMPR-sensitive nucleoside transporter in LLC-PK1 cells exhibited both a high affinity for cytidine (apparent K(i) approximately 65-mu-M for influx) and differential 'mobility' of the carrier (the kinetic parameters of equilibrium exchange of formycin B are greater than those for formycin B influx). An additional minor component of sodium-dependent uridine influx in LLC-PK, cells became detectable when the NBMPR-sensitive nucleoside transporter was blocked by the presence of 10-mu-M NBMPR. This active transport system was inhibited by adenosine, inosine and guanosine but thymidine and cytidine were without effect, inhibition properties identical to the N1 sodium-dependent nucleoside carrier in bovine renal outer cortical brush-border membrane vesicles (Williams and Jarvis (1991) Biochem. J. 274, 27-33). Late proximal tubule brush-border membrane vesicles of porcine kidney were shown to have a much reduced Na+-dependent uridine uptake activity compared to early proximal tubule porcine brush-border membrane vesicles. These results, together with the recent suggestion of thc late proximal tubular origin of LLC-PK1 cells, suggest that in vivo nucleoside transport across thc late proximal tubule cell may proceed mainly via a facilitated-diffusion process.
引用
收藏
页码:303 / 310
页数:8
相关论文
共 50 条
  • [11] METABOLISM OF L-LACTATE BY LLC-PK1 RENAL EPITHELIA
    MULLIN, JM
    CHA, CJM
    KLEINZELLER, A
    AMERICAN JOURNAL OF PHYSIOLOGY, 1982, 242 (01): : C41 - C45
  • [12] AMMONIA METABOLISM BY ROCKER CULTURED LLC-PK1 CELLS
    TANNEN, RL
    SCHEID, JM
    COLE, LA
    JOURNAL OF CLINICAL CHEMISTRY AND CLINICAL BIOCHEMISTRY, 1986, 24 (09): : 668 - 669
  • [13] CALCIUM HOMEOSTASIS IN CULTURED RENAL LLC-PK1 CELLS
    BONVENTRE, JV
    CHEUNG, JY
    FEDERATION PROCEEDINGS, 1985, 44 (03) : 626 - 626
  • [14] CHARACTERISTICS OF CYSTINE UPTAKE BY CULTURED LLC-PK1 CELLS
    FOREMAN, JW
    LEE, J
    SEGAL, S
    BIOCHIMICA ET BIOPHYSICA ACTA, 1988, 968 (03) : 323 - 330
  • [15] ORGANIC CATION-TRANSPORT IN CULTURED RENAL EPITHELIAL LLC-PK1 CELL MONOLAYERS
    DUDLEY, AJ
    BROWN, CDA
    JOURNAL OF PHYSIOLOGY-LONDON, 1994, 479P : P102 - P103
  • [16] EFFECTS OF APICAL VS BASOLATERAL PALYTOXIN ON LLC-PK1 RENAL EPITHELIA
    MULLIN, JM
    SNOCK, KV
    MCGINN, MT
    AMERICAN JOURNAL OF PHYSIOLOGY, 1991, 260 (06): : C1201 - C1211
  • [17] Glucuronidation and the transport of the glucuronide metabolites in LLC-PK1 cells
    Chang, Jae H.
    Benet, Leslie Z.
    MOLECULAR PHARMACEUTICS, 2005, 2 (05) : 428 - 434
  • [18] THE NATURE OF THE SIGNAL IN HEXOSE REGULATION OF SODIUM-DEPENDENT HEXOSE-TRANSPORT IN LLC-PK1 EPITHELIA
    MORAN, A
    TURNER, RJ
    HANDLER, JS
    JOURNAL OF GENERAL PHYSIOLOGY, 1983, 82 (06): : A14 - A15
  • [19] DIGOXIN TRANSPORT BY RENAL TUBULAR CELLS (LLC-PK1)
    ITO, S
    HARPER, PA
    KOREN, G
    PEDIATRIC RESEARCH, 1991, 29 (04) : A60 - A60
  • [20] BASOLATERAL TRANSPORT OF TETRAETHYLAMMONIUM BY A CLONE OF LLC-PK1 CELLS
    MCKINNEY, TD
    SCHELLER, MB
    HOSFORD, M
    LESNIAK, ME
    HASELEY, TS
    JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY, 1992, 2 (10): : 1507 - 1515