CONSTRUCTION AND SEROLOGICAL CHARACTERIZATION OF A RECOMBINANT HUMAN SINGLE-CHAIN T-CELL RECEPTOR

被引：14
|
作者：
LAKE, DF
BERNSTEIN, RM
HERSH, EM
KAYMAZ, H
SCHLUTER, SF
MARCHALONIS, JJ
机构：
[1] UNIV ARIZONA,COLL MED,TUCSON,AZ 85724
[2] ARIZONA CANC CTR,TUCSON,AZ
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 201卷 / 03期
关键词：
D O I：
10.1006/bbrc.1994.1874
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A single chain T cell receptor (scTcr) was constructed from the complete V alpha and V beta regions of Jurkat T-cell receptor alpha/beta chain genes using molecular cloning techniques. The recombinant scTcr reacted with a panel of rabbit antisera generated against synthetic 16-mer peptides duplicating the amino acid sequence of Jurkat V alpha and beta chains but not with antisera directed against peptides from the constant domain. Autoantibodies present in sera from systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA) patients bound the scTcr in ELISA assays. The recombinant scTcr described here should prove to be a useful reagent with which to study T-cell receptor activity in serological and functional assays. (C) 1994 Academic Press, Inc.
引用
收藏
页码：1502 / 1509
页数：8
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