Cultivation Milieu for Recombinant Strain Bacillus subtilis with Modified Gene of Glutamyl Endopeptidase of Bacillus intermedius

被引：0
|
作者：
Danilova, Yu. V. [1 ]
Balaban, N. P. [2 ,3 ]
Shamsutdinov, T. R. [4 ]
Mardanova, A. M. [2 ,5 ]
Sharipova, M. R. [2 ,6 ]
机构：
[1] Kazan Privolga Fed Univ, Dept Microbiol, Kazan, Russia
[2] Kazan Privolzhsky Fed Univ, Biol Sci, Kazan, Russia
[3] Kazan Privolzhsky Fed Univ, Dept Microbiol, Kazan, Russia
[4] Kazan Privolzhsky Fed Univ, Biol Sci Microbiol, Kazan, Russia
[5] Kazan Privolzhsky Fed Univ, Fedra Microbiol, Kazan, Russia
[6] Kazan Privolzhsky Fed Univ, Ka Fedra Microbiol, Kazan, Russia
来源：
UCHENYE ZAPISKI KAZANSKOGO UNIVERSITETA-SERIYA ESTESTVENNYE NAUKI | 2011年 / 153卷 / 02期
关键词：
B; subtilis; intermedius; recombinant strain; biosynthesis; glutamyl endopeptidase;
D O I：
暂无
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
It is known that proteinase biosynthesis is influenced by the conditions of a producer's cultivation. We show that enzyme production is 5 times higher on the medium with pH 7.7 than on the medium with pH 8.5 traditionally used for biosynthesis of many hydrolytic enzymes, including proteinases of initial strain of B. intermedius 3-19 and recombinant strains of B. subtilis AJ73. Maximum efficiency of recombinant strain in terms of the synthesis of glutamyl endopeptidase is observed with a medium volume to flask volume ratio of 1 : 4.
引用
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页码：51 / 62
页数：3
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