The cement produced microcrystals of calcite by reaction with culture medium supplemented with calf serum. Human dental pulp cells seeded on such a substrate preferentially adhered and aggregated around the microcrystals. Immunofluorescence and immunogold labelling revealed a high affinity of serum fibronectin molecules for the calcite crystals. At 4 weeks in culture, the cells had various features of differentiated odontoblasts, notably nuclear polarization, typical appearance of the Golgi apparatus, synthesis of type I collagen and absence of type III, and apical accumulation of actin and vimentin. These cells also elaborated a collagenous extracellular matrix which did not mineralize.
机构:
Univ Lyon, F-69000 Lyon, France
Inst Genom Fonct Lyon, F-69007 Lyon, France
IGFL, CNRS, UMR 5242, F-69007 Lyon, France
Univ Lyon 1, F-69100 Villeurbanne, France
ENSL, F-69007 Lyon, France
INRA, UMR 1288, F-69007 Lyon, France
INSERM, ERI 16, Fac Odontol, F-69008 Lyon, FranceUniv Oulu, Inst Dent, Oulu 90014, Finland
Bleicher, Francoise
Carrouel, Florence
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机构:
Univ Lyon, F-69000 Lyon, France
Inst Genom Fonct Lyon, F-69007 Lyon, France
IGFL, CNRS, UMR 5242, F-69007 Lyon, France
Univ Lyon 1, F-69100 Villeurbanne, France
ENSL, F-69007 Lyon, France
INRA, UMR 1288, F-69007 Lyon, France
INSERM, ERI 16, Fac Odontol, F-69008 Lyon, FranceUniv Oulu, Inst Dent, Oulu 90014, Finland
机构:
Int Ctr Life, Inst Human Genet, Newcastle Upon Tyne, Tyne & Wear, EnglandUniv Oulu, Inst Dent, Oulu 90014, Finland
Wappler, Ilka
Couble, Marie-Lise
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机构:
Univ Lyon, F-69000 Lyon, France
Inst Genom Fonct Lyon, F-69007 Lyon, France
IGFL, CNRS, UMR 5242, F-69007 Lyon, France
Univ Lyon 1, F-69100 Villeurbanne, France
ENSL, F-69007 Lyon, France
INRA, UMR 1288, F-69007 Lyon, France
INSERM, ERI 16, Fac Odontol, F-69008 Lyon, FranceUniv Oulu, Inst Dent, Oulu 90014, Finland
Couble, Marie-Lise
Magloire, Henry
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机构:
Univ Lyon, F-69000 Lyon, France
Inst Genom Fonct Lyon, F-69007 Lyon, France
IGFL, CNRS, UMR 5242, F-69007 Lyon, France
Univ Lyon 1, F-69100 Villeurbanne, France
ENSL, F-69007 Lyon, France
INRA, UMR 1288, F-69007 Lyon, France
INSERM, ERI 16, Fac Odontol, F-69008 Lyon, FranceUniv Oulu, Inst Dent, Oulu 90014, Finland
Magloire, Henry
Peters, Heiko
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Int Ctr Life, Inst Human Genet, Newcastle Upon Tyne, Tyne & Wear, EnglandUniv Oulu, Inst Dent, Oulu 90014, Finland
机构:
Capital Med Univ, Beijing Stomatol Hosp, Dept Orthodont, Tian Tan Xi Li 4, Beijing 100050, Peoples R ChinaCapital Med Univ, Beijing Stomatol Hosp, Dept Orthodont, Tian Tan Xi Li 4, Beijing 100050, Peoples R China
Gu, Yingzhi
Zhuang, Rui
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机构:
Capital Med Univ, Beijing Stomatol Hosp, Dept Oral & Maxillofacial Surg, Beijing, Peoples R ChinaCapital Med Univ, Beijing Stomatol Hosp, Dept Orthodont, Tian Tan Xi Li 4, Beijing 100050, Peoples R China
Zhuang, Rui
Xie, Xianju
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Capital Med Univ, Beijing Stomatol Hosp, Dept Orthodont, Tian Tan Xi Li 4, Beijing 100050, Peoples R ChinaCapital Med Univ, Beijing Stomatol Hosp, Dept Orthodont, Tian Tan Xi Li 4, Beijing 100050, Peoples R China
Xie, Xianju
Bai, Yuxing
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Capital Med Univ, Beijing Stomatol Hosp, Dept Orthodont, Tian Tan Xi Li 4, Beijing 100050, Peoples R ChinaCapital Med Univ, Beijing Stomatol Hosp, Dept Orthodont, Tian Tan Xi Li 4, Beijing 100050, Peoples R China
机构:
Fourth Mil Med Univ, Dept Prosthodont, Sch Stomatol, State Key Lab Mil Stomatol, Xian 710032, Shaanxi, Peoples R China
Georgia Regents Univ, Dept Endodont, Augusta, GA USAFourth Mil Med Univ, Dept Prosthodont, Sch Stomatol, State Key Lab Mil Stomatol, Xian 710032, Shaanxi, Peoples R China
Niu, Li-na
Watson, Devon
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机构:
Georgia Regents Univ, Dept Endodont, Augusta, GA USAFourth Mil Med Univ, Dept Prosthodont, Sch Stomatol, State Key Lab Mil Stomatol, Xian 710032, Shaanxi, Peoples R China
Watson, Devon
Thames, Kyle
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机构:
Georgia Regents Univ, Dept Endodont, Augusta, GA USAFourth Mil Med Univ, Dept Prosthodont, Sch Stomatol, State Key Lab Mil Stomatol, Xian 710032, Shaanxi, Peoples R China
Thames, Kyle
Primus, Carolyn M.
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机构:
LECOM Sch Dent Med, Bradenton, FL USAFourth Mil Med Univ, Dept Prosthodont, Sch Stomatol, State Key Lab Mil Stomatol, Xian 710032, Shaanxi, Peoples R China
Primus, Carolyn M.
Bergeron, Brian E.
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机构:
Georgia Regents Univ, Dept Endodont, Augusta, GA USAFourth Mil Med Univ, Dept Prosthodont, Sch Stomatol, State Key Lab Mil Stomatol, Xian 710032, Shaanxi, Peoples R China
Bergeron, Brian E.
Jiao, Kai
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机构:
Fourth Mil Med Univ, Dept Prosthodont, Sch Stomatol, State Key Lab Mil Stomatol, Xian 710032, Shaanxi, Peoples R ChinaFourth Mil Med Univ, Dept Prosthodont, Sch Stomatol, State Key Lab Mil Stomatol, Xian 710032, Shaanxi, Peoples R China
Jiao, Kai
Bortoluzzi, Eduardo A.
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机构:
Univ Fed Santa Catarina, Dept Dent, Florianopolis, SC, BrazilFourth Mil Med Univ, Dept Prosthodont, Sch Stomatol, State Key Lab Mil Stomatol, Xian 710032, Shaanxi, Peoples R China
Bortoluzzi, Eduardo A.
Cutler, Christopher W.
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机构:
Georgia Regents Univ, Dept Periodont, Augusta, GA USAFourth Mil Med Univ, Dept Prosthodont, Sch Stomatol, State Key Lab Mil Stomatol, Xian 710032, Shaanxi, Peoples R China
Cutler, Christopher W.
Chen, Ji-hua
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h-index: 0
机构:
Fourth Mil Med Univ, Dept Prosthodont, Sch Stomatol, State Key Lab Mil Stomatol, Xian 710032, Shaanxi, Peoples R ChinaFourth Mil Med Univ, Dept Prosthodont, Sch Stomatol, State Key Lab Mil Stomatol, Xian 710032, Shaanxi, Peoples R China
Chen, Ji-hua
Pashley, David H.
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机构:
Georgia Regents Univ, Dept Oral Biol, Augusta, GA USAFourth Mil Med Univ, Dept Prosthodont, Sch Stomatol, State Key Lab Mil Stomatol, Xian 710032, Shaanxi, Peoples R China
Pashley, David H.
Tay, Franklin R.
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h-index: 0
机构:
Georgia Regents Univ, Dept Endodont, Augusta, GA USAFourth Mil Med Univ, Dept Prosthodont, Sch Stomatol, State Key Lab Mil Stomatol, Xian 710032, Shaanxi, Peoples R China