ODONTOBLAST-LIKE CYTODIFFERENTIATION OF HUMAN DENTAL-PULP CELLS-INVITRO IN THE PRESENCE OF A CALCIUM HYDROXIDE-CONTAINING CEMENT

被引:104
|
作者
SEUX, D
COUBLE, ML
HARTMANN, DJ
GAUTHIER, JP
MAGLOIRE, H
机构
[1] INST PASTEUR, CTR RADIOANAL, LYON, FRANCE
[2] LAB MINERAL CRISTALLOG, F-69622 VILLEURBANNE 03, FRANCE
关键词
ODONTOBLAST; CYTODIFFERENTIATION; INVITRO; CA(OH)2; IMMUNOLABELING; X-RAY DIFFRACTION;
D O I
10.1016/0003-9969(91)90074-5
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
The cement produced microcrystals of calcite by reaction with culture medium supplemented with calf serum. Human dental pulp cells seeded on such a substrate preferentially adhered and aggregated around the microcrystals. Immunofluorescence and immunogold labelling revealed a high affinity of serum fibronectin molecules for the calcite crystals. At 4 weeks in culture, the cells had various features of differentiated odontoblasts, notably nuclear polarization, typical appearance of the Golgi apparatus, synthesis of type I collagen and absence of type III, and apical accumulation of actin and vimentin. These cells also elaborated a collagenous extracellular matrix which did not mineralize.
引用
收藏
页码:117 / 128
页数:12
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