DIFFERENTIAL-EFFECTS OF HISTIDINE ON HYDROGEN PEROXIDE-INDUCED BACTERIAL KILLING AND DNA NICKING IN-VITRO

The hydrogen peroxide dose-response curves for Escherichia coli killing and DNA nicking in vitro display remarkably similar bimodal patterns. The concentrations of the oxidant resulting in maximum mode one killing, however, exceeds by two orders of magnitude those resulting in the mode one DNA nicking response. Addition of histidine differentially affects the experimental curves describing the dose-dependency for bacterial killing and DNA damage in vitro. Indeed, the lethal effect elicited by the oxidant in the presence of the amino acid is strictly concentration-dependent and thus the inactivation curve loses its bimodal character. In marked contrast, histidine abolishes DNA damage generated by low concentrations of hydrogen peroxide(< 100 mu M) in the in vitro system (the mode one DNA nicking response) but greatly increases DNA damage produced by concentrations of the oxidant higher than 1 mM (the mode two DNA nicking response). Experimental results also suggest that treatment of covalently closed circular double-stranded supercoiled DNA with hydrogen peroxide, in the presence of both histidine and iron, may result in the formation of DNA double strand breakage, a type of lesion which is not efficiently produced by the oxidant in the absence of the amino acid. Taken together, the above results indicate that histidine differentially affects the in vitro DNA cleavage and E. coli lethality induced by hydrogen peroxide and suggest that different molecular events mediate mode one DNA nicking in vitro and mode one killing of bacterial cells.