AFFINITY PURIFICATION OF SYNTHETIC PEPTIDES AND PROTEINS ON POROUS GRAPHITIZED CARBON

被引:28
|
作者
BROWN, AR [1 ]
IRVING, SL [1 ]
RAMAGE, R [1 ]
机构
[1] UNIV EDINBURGH,DEPT CHEM,W MAINS RD,EDINBURGH EH9 3JJ,MIDLOTHIAN,SCOTLAND
关键词
AFFINITY CHROMATOGRAPHY; TBFMOC N-ALPHA-PROTECTION; POROUS GRAPHITIZED CARBON; TETRABENZO[A; C; G; I]FLUORENYL-17-METHANOL; PROTEIN PURIFICATION;
D O I
10.1016/S0040-4039(00)61617-9
中图分类号
O62 [有机化学];
学科分类号
070303 ; 081704 ;
摘要
The affinity of the Tbfmoc group for porous graphitised carbon has been exploited for the purification of synthetic peptides containing up to 85 amino acids. The hydrophobicity of the group has also been used to simplify peptide purification by HPLC.
引用
收藏
页码:7129 / 7132
页数:4
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