AFFINITY PURIFICATION OF SYNTHETIC PEPTIDES AND PROTEINS ON POROUS GRAPHITIZED CARBON

被引：28
|
作者：
BROWN, AR [1 ]
IRVING, SL [1 ]
RAMAGE, R [1 ]
机构：
[1] UNIV EDINBURGH,DEPT CHEM,W MAINS RD,EDINBURGH EH9 3JJ,MIDLOTHIAN,SCOTLAND
来源：
TETRAHEDRON LETTERS | 1993年 / 34卷 / 44期
关键词：
AFFINITY CHROMATOGRAPHY; TBFMOC N-ALPHA-PROTECTION; POROUS GRAPHITIZED CARBON; TETRABENZO[A; C; G; I]FLUORENYL-17-METHANOL; PROTEIN PURIFICATION;
D O I：
10.1016/S0040-4039(00)61617-9
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
The affinity of the Tbfmoc group for porous graphitised carbon has been exploited for the purification of synthetic peptides containing up to 85 amino acids. The hydrophobicity of the group has also been used to simplify peptide purification by HPLC.
引用
收藏
页码：7129 / 7132
页数：4
相关论文
共 50 条
  • [1] AFFINITY PURIFICATION OF SYNTHETIC PEPTIDES
    KRIEGER, DE
    ERICKSON, BW
    MERRIFIELD, RB
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1976, 73 (09) : 3160 - 3164
  • [2] FUNCTIONAL PURIFICATION OF PROTEINS AND PEPTIDES BY AFFINITY CHROMATOGRAPHY
    CUATRECA.P
    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1971, 19 (04) : 600 - &
  • [3] Anomalous retention behaviour of peptides on porous graphitized carbon column
    NemethKiss, V
    Forgacs, E
    Cserhati, T
    JOURNAL OF CHROMATOGRAPHY A, 1997, 776 (01) : 147 - 152
  • [4] FUNCTIONAL PURIFICATION OF PROTEINS AND PEPTIDES BY AFFINITY CHROMATOGRAPHY - AGFD
    CUATRECA.P
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 1970, (SEP): : 30 - &
  • [5] AFFINITY PURIFICATION SYSTEM FOR RECOMBINANT PEPTIDES PROTEINS BASED ON A CALMODULIN FUSION TAIL
    VUKASINOVIC, V
    HENTZ, NG
    DAUNERT, S
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 1995, 209 : 72 - ANYL
  • [6] Affinity purification of recombinant proteins fused to calmodulin or to calmodulin-binding peptides
    Vaillancourt, P
    Zheng, CF
    Hoang, DQ
    Briester, L
    APPLICATIONS OF CHIMERIC GENES AND HYBRID PROTEINS, PT A, 2000, 326 : 340 - 362
  • [7] PURIFICATION OF SYNTHETIC PEPTIDES - IMMOBILIZED METAL-ION AFFINITY-CHROMATOGRAPHY (IMAC)
    LINDEBERG, G
    BENNICH, H
    ENGSTROM, A
    INTERNATIONAL JOURNAL OF PEPTIDE AND PROTEIN RESEARCH, 1991, 38 (03): : 253 - 259
  • [8] PURIFICATION OF SYNTHETIC PROTEINS
    WOO, DDL
    CLARKELEWIS, I
    KENT, SBH
    JOURNAL OF CELLULAR BIOCHEMISTRY, 1987, : 165 - 165
  • [9] Peptides as affinity surfaces for protein purification
    Pingali, A
    McGuinness, B
    Keshishian, H
    Jin, FW
    Varady, L
    Regnier, F
    JOURNAL OF MOLECULAR RECOGNITION, 1996, 9 (5-6) : 426 - 432
  • [10] Carbon nanotubes as affinity probes for peptides and proteins in MALDI MS analysis
    Chen, WY
    Wang, LS
    Chiu, HT
    Chen, YC
    Lee, CY
    JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 2004, 15 (11) : 1629 - 1635
12345