TRIGONAL CRYSTALS OF PORCINE MITOCHONDRIAL ASPARTATE-AMINOTRANSFERASE

被引：7
|
作者：
IZARD, T
FOL, B
PAUPTIT, RA
JANSONIUS, JN
机构：
[1] Department of Structural Biology Biocentre, University of Basel, Basel, CH-4056
来源：
JOURNAL OF MOLECULAR BIOLOGY | 1990年 / 215卷 / 03期
关键词：
D O I：
10.1016/S0022-2836(05)80355-9
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Crystals suitable for X-ray analysis of procine mitochondrial aspartate aminotransferase in the closed conformation were obtained after the apoenzyme was reconstituted with N-5′-phosphopyridoxyl-l-aspartate, an inhibitor in which the cofactor is covalently bound to the substrate. This results in a crystal form that has not been encountered previously in studies of aspartate aminotransferases. The crystals belong to the trigonal space group P3121 (or the enantiomeric P3221) with unit cell dimensions a=b=202·0 , c=58·0 , α=β=900, ψ=1200 and contain one dimer in the asymmetric unit. © 1990, Academic Press Limited. All rights reserved.
引用
收藏
页码：341 / 344
页数：4
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