ISOLATION AND PURIFICATION OF CALMODULIN FROM THE SHRIMP, CRANGON-CRANGON

被引：9
|
作者：
MICHAEL, RH
PIPKORN, R
WILLIG, A
JAROS, PP
机构：
[1] UNIV OLDENBURG, FACHBEREICH BIOL, DEPT ZOOPHYSIOL, POB 2503, W-2900 OLDENBURG, GERMANY
[2] UNIV HEIDELBERG, ZENTRUM MOLEK BIOL, W-6900 HEIDELBERG, GERMANY
来源：
LIFE SCIENCES | 1992年 / 51卷 / 24期
关键词：
D O I：
10.1016/0024-3205(92)90040-V
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
Calmodulin was isolated and purified from shrimp abdominal muscle by heat precipitation, ion exchange and hydrophobic interaction chromatography. The purified calmodulin was homogeneous when evaluated by polyacrylamide gel electrophoresis. A still remaining contaminant was eliminated by high performance liquid chromatography on a phenyl column. The biological and physicochemical properties of shrimp calmodulin such as amino acid composition, molecular weight and the ability to activate calmodulin-deficient bovine heart phosphodiesterase were compared to those of other invertebrate calmodulins.
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页码：1881 / 1889
页数：9
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