Waste activated sludge was thickened by biological flotation without polymer flocculant dosage. The BIOFLOT(R) process utilizes the denitrifying ability of activated sludge bacteria. Gaseous products of anaerobic nitrate reduction cause spontaneous flotation of the sludge suspended solids. Laboratory tests confirmed the dependence of sludge thickening efficiency on available nitrate concentration, flotation time and temperature. Full-scale experiments were performed in a fully automatized unit for discontinuous sludge thickening from wastewater treatment plants with a capacity of up to 5000 I.E. Waste activated sludge from wastewater treatment plants at Pisek, Milevsko and Bjornlunda was thickened from 6.2, 10.7 and 3.5 g/l MLSS to 59.4, 59.7 and 66.7 g/l MLSS, respectively. Concentrations of COD, ammonium and phosphate ions were decreased in sludge water. The average nitrate consumption for bioflotation was 21.2 mg NO3- per 1 g of MLSS of activated sludge. Flotation time ranged from 4 to 48 h.