Enzymatic Hydrolysis of Palm Stearin to Produce Monoglyceride by Lipase from Rhizomucor miehei and Pancreatic

The objectives of the research were to evaluate the effect of the pH, ratio of substrate: phospate buffer, and reaction time on the enzymatic hydrolysis of palm stearin to obtain monoglyceride by R. miehei and pancreatic lipases. Hydrolysis was evaluated at various pH (6.0; 6.5; 7.0; 7.5 dan 8.0). Enzymatic hydrolysis reactions were held at various ratio of substrate: phospate buffer (10: 1, 10: 2, 10: 3, 10: 4, 10: 5, 10: 6) and duration time of 6, 12, 18, 24 hours by R. miehei lipase and 24, 30, 36, 42, 48 hours by pancreatic lipase. Enzymatic hydrolysis reaction was carried out in waterbath shaker 80 stroke/minute, at 40 degrees C with R. miehei lipase and 37 degrees C with pancreatic lipase. The hydrolysis products were monitored using TLC with petroleum ether: diethyl ether: acetic acid=60: 40: 1 as developing solvent on silica gel F254 20x20 cm plate. The results showed that optimum pH for both R. miehei and pancreatic lipases were 6.5 and their activities were 332.25 unit/g enzyme amobile and 228.04 unit/g enzyme, respectively. The highest monoglyceride fraction was obtained from ratio substrate: phospate buffer 10: 1 at 18 hours of incubation by Rhizomucor miehei lipase (21,59%) and ratio substrate: phospate buffer 10: 4 at 42 hours of incubation by pancreatic lipase (40,45%).