AUTOMATED HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE DETERMINATION OF NEDOCROMIL SODIUM IN HUMAN URINE USING BIMODAL COLUMN-SWITCHING

被引：4
|
作者：
BAKER, PR
GARDNER, JJ
WILKINSON, D
机构：
[1] FISONS PLC,DIV PHARMACEUT,RES & DEV LABS,DEPT PHYS CHEM,LOUGHBOROUGH LE11 0RH,LEICS,ENGLAND
[2] CLIN INNOVAT LTD,KENILWORTH CV8 2LY,WARWICK,ENGLAND
来源：
JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1995年 / 668卷 / 01期
关键词：
D O I：
10.1016/0378-4347(95)00049-O
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
An automated HPLC method is described for the determination of nedocromil sodium in human urine. An HPLC autosampler is used to inject urine samples onto a short reversed-phase column. This column acts as a concentration column and performs a preliminary extraction. The concentration column is automatically back-flushed onto an ion-exchange column where final separation of nedocromil sodium from urine constituents occurs. Recovery, accuracy, precision, sensitivity and specificity were investigated. The method has been applied to urine samples from clinical studies, and the results were compared to those obtained using a radioimmunoassay developed previously.
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页码：59 / 65
页数：7
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