Stable-isotope Labeled Metabolic Analysis in Drosophila melanogaster: From Experimental Setup to Data Analysis

被引：1
|
作者：
Cai, Yuping [1 ]
Liu, Nan [1 ]
Zhu, Zheng-Jiang [1 ]
机构：
[1] Chinese Acad Sci, Interdisciplinary Res Ctr Biol & Chem, Shanghai Inst Organ Chem, Shanghai, Peoples R China
来源：
BIO-PROTOCOL | 2018年 / 8卷 / 18期
关键词：
Stable-isotope labeling; C-13-glucose tracer; Metabolic analysis; Qualitative analysis; Liquid chromatography-mass spectrometry; Drosophila melanogaster;
D O I：
10.21769/BioProtoc.3015
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Stable-isotope labeled metabolic analysis is an essential methodology to characterize metabolic regulation during biological processes. However, the method using stable-isotope-labeled tracer (e.g., C-13-glucose) in live animal is only beginning to be developed. Here, we contribute a qualitative metabolic labeling experiment protocol in Drosophila melanogaster using stable-isotope-labeled C-13-glucose tracer followed by liquid chromatography-mass spectrometry (LC-MS) analysis. Detailed experimental setup, data acquisition and analysis are provided to facilitate the application of in vivo metabolic labeling analysis that might be applied in a wide range of biological studies.
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页数：9
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