SYNTHESIS OF A GENE FOR RAT-LIVER FATTY-ACID-BINDING PROTEIN AND ITS EXPRESSION IN ESCHERICHIA-COLI

被引：40
|
作者：
WORRALL, AF
EVANS, C
WILTON, DC
机构：
[1] UNIV SOUTHAMPTON,DEPT BIOCHEM,SOUTHAMPTON SO9 3TU,HANTS,ENGLAND
[2] UNIV SOUTHAMPTON,SERC,CTR MOLEC RECOGNIT,SOUTHAMPTON SO9 3TU,HANTS,ENGLAND
来源：
BIOCHEMICAL JOURNAL | 1991年 / 278卷
基金：
英国惠康基金;
关键词：
D O I：
10.1042/bj2780365
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A gene coding for rat liver fatty-acid-binding protein (FABP) has been constructed by the single-step ligation of ten synthetic oligonucleotides. The gene has been cloned into the bacterial expression vector pKK223-3. Induction of protein synthesis from this gene results in over expression of an FABP that is indistinguishable in its structure and binding properties with that isolated from rat liver.
引用
收藏
页码：365 / 368
页数：4
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