SYNTHESIS OF A GENE FOR RAT-LIVER FATTY-ACID-BINDING PROTEIN AND ITS EXPRESSION IN ESCHERICHIA-COLI

被引：40
|
作者：
WORRALL, AF
EVANS, C
WILTON, DC
机构：
[1] UNIV SOUTHAMPTON,DEPT BIOCHEM,SOUTHAMPTON SO9 3TU,HANTS,ENGLAND
[2] UNIV SOUTHAMPTON,SERC,CTR MOLEC RECOGNIT,SOUTHAMPTON SO9 3TU,HANTS,ENGLAND
来源：
BIOCHEMICAL JOURNAL | 1991年 / 278卷
基金：
英国惠康基金;
关键词：
D O I：
10.1042/bj2780365
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A gene coding for rat liver fatty-acid-binding protein (FABP) has been constructed by the single-step ligation of ten synthetic oligonucleotides. The gene has been cloned into the bacterial expression vector pKK223-3. Induction of protein synthesis from this gene results in over expression of an FABP that is indistinguishable in its structure and binding properties with that isolated from rat liver.
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页码：365 / 368
页数：4
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